Jul 1, 1982

Carbon balance studies of glucose metabolism in rat cerebral cortical synaptosomes

Journal of Neurochemistry
U Bauer, K Brand

Abstract

Synaptosomes were isolated from rat cerebral cortex and incubated with [U-14C]-, [1-14C]- or [6-14C]glucose. Glucose utilization and the metabolic partitioning of glucose carbon in products were determined by isotopic methods. From the data obtained a carbon balance was constructed, showing lactate to be the main product of glucose metabolism, followed by CO2, amino acids and pyruvate. Measuring the release of 14CO2 from glucose labelled in three different positions allowed the construction of a flow diagram of glucose carbon atoms in synaptosomes, which provides information about the contribution of the various pathways of glucose metabolism. Some 2% of glucose utilized was calculated to be degraded via the pentose phosphate pathway. Addition of chlorpromazine, imipramine or haloperidol at concentrations of 10(-5) M reduced glucose utilisation by 30% without changing the distribution pattern of radioactivity in the various products.

  • References12
  • Citations6

References

Mentioned in this Paper

Metabolic Process, Cellular
Biochemical Pathway
Pentose Phosphate Pathway
Assay OF Haloperidol
Lactate
Amino Acids, I.V. solution additive
August Rats
Pyruvate Measurement
Brain
Pentosephosphates

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