Carbon-based hierarchical scaffolds for myoblast differentiation: Synergy between nano-functionalization and alignment

Acta Biomaterialia
Akhil PatelShilpa Sant

Abstract

While several scaffolds have been proposed for skeletal muscle regeneration, multiscale hierarchical scaffolds with the complexity of extracellular matrix (ECM) haven't been engineered successfully. By precise control over nano- and microscale features, comprehensive understanding of the effect of multiple factors on skeletal muscle regeneration can be derived. In this study, we engineered carbon-based scaffolds with hierarchical nano- and microscale architecture with controlled physico-chemical properties. More specifically, we built multiscale hierarchy by growing carbon nanotube (CNT) carpets on two types of scaffolds, namely, interconnected microporous carbon foams and aligned carbon fiber mats. Nanostructured CNT carpets offered fine control over nano-roughness and wettability facilitating myoblast adhesion, growth and differentiation into myocytes. However, microporous foam architecture failed to promote their fusion into multinucleated myotubes. On the other hand, aligned fibrous architecture stimulated formation of multinucleated myotubes. Most importantly, nanostructured CNT carpets interfaced with microscale aligned fibrous architecture significantly enhanced myocyte fusion into multinucleated mature myotubes highligh...Continue Reading

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Citations

Jun 22, 2016·Journal of Materials Science. Materials in Medicine·Klaire WilsonJeffrey C Wolchok
Oct 1, 2016·Journal of Tissue Engineering and Regenerative Medicine·Sharanya SankarSeeram Ramakrishna
May 30, 2017·Nanomedicine : Nanotechnology, Biology, and Medicine·Ozlem ErolMustafa O Guler
Mar 18, 2016·Annals of Biomedical Engineering·Akhil PatelShilpa Sant
Mar 9, 2018·Beilstein Journal of Nanotechnology·Razieh BeigmoradiDavod Mohebbi-Kalhori
Jul 22, 2020·Advanced Healthcare Materials·Amy Corbin FarrAntonios G Mikos
Feb 23, 2018·Journal of Biomaterials Applications·Gabriele BöhmUrsula Mirastschijski
Jul 14, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jianyong FengChangyou Gao
Jan 10, 2021·International Journal of Molecular Sciences·Kamal AwadVenu Varanasi
Jan 12, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Soham D ParikhDebra A Mayes
Sep 1, 2019·Regenerative Engineering and Translational Medicine·Xiaoyan TangCato T Laurencin
Jun 3, 2021·International Journal of Molecular Sciences·Emine AlarcinMeltem Avci-Adali
Jun 22, 2021·Journal of Materials Science. Materials in Medicine·Selva BilgePinar Yilgor Huri

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