Carbon catabolite repression correlates with the maintenance of near invariant molecular crowding in proliferating E. coli cells

BMC Systems Biology
Yi ZhouZ N Oltvai

Abstract

Carbon catabolite repression (CCR) is critical for optimal bacterial growth, and in bacterial (and yeast) cells it leads to their selective consumption of a single substrate from a complex environment. However, the root cause(s) for the development of this regulatory mechanism is unknown. Previously, a flux balance model (FBAwMC) of Escherichia coli metabolism that takes into account the crowded intracellular milieu of the bacterial cell correctly predicted selective glucose uptake in a medium containing five different carbon sources, suggesting that CCR may be an adaptive mechanism that ensures optimal bacterial metabolic network activity for growth. Here, we show that slowly growing E. coli cells do not display CCR in a mixed substrate culture and gradual activation of CCR correlates with an increasing rate of E. coli cell growth and proliferation. In contrast, CCR mutant cells do not achieve fast growth in mixed substrate culture, and display differences in their cell volume and density compared to wild-type cells. Analyses of transcriptome data from wt E. coli cells indicate the expected regulation of substrate uptake and metabolic pathway utilization upon growth rate change. We also find that forced transient increase of i...Continue Reading

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Citations

Apr 19, 2015·BMC Systems Biology·John A ColeZaida Luthey-Schulten
Aug 4, 2016·Scientific Reports·Alexei Vazquez, Zoltán N Oltvai
Mar 28, 2017·Nature Reviews. Microbiology·Jonas van den BergBert Poolman
Dec 1, 2017·Journal of the Royal Society, Interface·Hidde de JongJean-Luc Gouzé
Mar 2, 2016·Scientific Reports·Avlant Nilsson, Jens Nielsen

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Datasets Mentioned

BETA
GSE51581

Methods Mentioned

BETA
protein folding
PCR
chips

Software Mentioned

ImageJ
qspline
Invitrogen®
FBAwMC
dchip
OxPhos
R

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