PMID: 8606180Apr 1, 1996Paper

Carbon catabolite repression of phenol degradation in Pseudomonas putida is mediated by the inhibition of the activator protein PhlR

Journal of Bacteriology
C MüllerH Herrmann

Abstract

Enzymes involved in (methyl)phenol degradation of Pseudomonas putida H are encoded by the catabolic operon (phlA-L) on plasmid pPGH1. Transcription of this operon by the sigma54 (RpoN)-containing RNA polymerase is positively controlled by the gene product of the divergently transcribed phlR in response to the availability of the respective substrate. Additionally, phenol degradation is subject to carbon catabolite repression induced by organic acids (e.g., succinate, lactate, and acetate) or carbohydrates (e.g., glucose and gluconate). Analysis of lacZ fusion to the catabolic promoter and quantified primer extension experiments indicate that carbon catabolite repression also occurs at the transcriptional level of the catabolic operon. In this study, it is furthermore shown that carbon catabolite repression is a negative control. Titration of the postulated negative controlling factor was exclusively observed when extra copies of functional phlR gene were present in the cell. We therefore conclude that PhlR is the target and that carbon catabolite repression of phenol degradation occurs by interfering with the activating function of PhlR.

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Citations

Mar 1, 2003·Archives of Microbiology·Avinash SonawaneKlaus-Heinrich Röhm
Jun 10, 2004·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Inna P Solyanikova, Ludmila A Golovleva
Oct 24, 2002·FEMS Microbiology Letters·Lothar PetruschkaHeidrun Herrmann
May 15, 2004·Environmental Microbiology·Jeremy A RentzJerald L Schnoor
Jul 17, 2008·Nature Reviews. Microbiology·Boris Görke, Jörg Stülke
Mar 24, 2016·International Journal of Environmental Research and Public Health·Zhenghui LiuShusi Liu
Sep 22, 2007·Omics : a Journal of Integrative Biology·Prashant S PhaleRahul Shrivastava
Jun 19, 2014·Applied Microbiology and Biotechnology·Juraj SzőkölMiroslav Pátek
Aug 22, 2015·Genome Announcements·Paula VizosoIgnacio Poblete-Castro
Nov 27, 2019·Cellular and Molecular Life Sciences : CMLS·Heejoon ParkRoss P Carlson
Aug 14, 2020·Applied Microbiology and Biotechnology·Anna WeimerChristoph Wittmann
Dec 17, 2002·The Journal of General and Applied Microbiology·Vera L. Santos, Valter R. Linardi
Nov 5, 2002·FEMS Microbiology Reviews·Niall D O'LearyAlan D W Dobson
Feb 16, 2006·Environmental Microbiology·Leonid KurbatovLothar Petruschka
Aug 30, 2002·Applied and Environmental Microbiology·Jung-Kul LeeSang-Yong Kim
Jan 9, 2004·Applied and Environmental Microbiology·Christoph WerlenJan Roelof van der Meer
Mar 5, 2009·Microbiology and Molecular Biology Reviews : MMBR·Manuel CarmonaEduardo Díaz
Jan 5, 2000·Applied and Environmental Microbiology·A A Wise, C R Kuske
Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·David Tropel, Jan Roelof van der Meer

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