Carbon dots as an "on-off-on" fluorescent probe for detection of Cu(II) ion, ascorbic acid, and acid phosphatase

Analytical and Bioanalytical Chemistry
Xin MaFan Xia

Abstract

Carbon dot (CD)-based fluorescent probes have been widely exploited; however, multi-component detection using CDs without tedious surface modification is always a challenging task. Here, we develop a convenient and simple CD-based "on-off-on" fluorescent probe for detection of copper(II) ion (Cu2+), ascorbic acid (AA), and acid phosphatase (ACP). Cu2+ leads to the fluorescence quenching of CDs. The limit of detection (LOD) for Cu2+ is 2.4 μM. When AA is added into the CDs + Cu2+ solution, Cu2+ is reduced by AA to Cu+, causing the fluorescence recovery of CDs. The fluorescent intensity linearly correlates with the concentration of AA in the range of 100-2800 μM with LOD of 60 μM. Besides, the probe has potential application for detection of AA in real samples such as VC tablets, orange juice, and fresh orange. The probe can also indirectly detect ACP that enzymatically hydrolyzes ascorbic acid-phosphate (AAP) to produce AA. This work expands the application of CDs in the multi-component detection and provides a facile fluorescent probe for detection of AA in real samples. Graphical abstract.

References

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Jul 25, 2014·Biosensors & Bioelectronics·Yongming GuoMingchao Zhang
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Citations

Dec 15, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Eduardo Luiz RossiniHelena Redigolo Pezza
Oct 10, 2020·Biosensors & Bioelectronics·Yangxia HanHongdeng Qiu
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tengfei WangYu Wang
Dec 11, 2021·Analytical Methods : Advancing Methods and Applications·Hao QiShuo Wang

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