Carbon dots for fluorescent detection of α-glucosidase activity using enzyme activated inner filter effect and its application to anti-diabetic drug discovery

Analytica Chimica Acta
Weiheng KongYongning Wu

Abstract

Recently, α-glucosidase inhibitor has been widely used in clinic for diabetic therapy. In the present study, a facile and sensitive fluorescent assay based on enzyme activated inner filter effect (IFE) on nitrogen-doped carbon dots (CDs) was first developed for the detection of α-glucosidase. The N-doped CDs with green emission were prepared by a one-step hydrothermal synthesis and gave the fluorescence quantum yield of 30%, which were used as the signal output. Through α-glucosidase catalysis, 4-nitrophenol was released from 4-nitrophenyl-α-d-glucopyranoside (NGP). Interestingly, the absorption of 4-nitrophenol and the excitation of CDs were completely overlapping. Due to its great molar absorptivity, 4-nitrophenol was capable of acting as a powerful absorber to affect the fluorescent signal of CDs (i.e. IFE). By converting the absorption signals into fluorescence signals, the facile fluorescence assay strategy could be realized for α-glucosidase activity sensing, which effectively avoided the complex modification of the surface of CDs or construction of the nanoprobes. The established IFE-based sensing platform offered a low detection limit of 0.01 U/mL (S/N = 3). This proposed sensing approach has also been expanded to the i...Continue Reading

Citations

Nov 25, 2017·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Hui Feng, Zhaosheng Qian
Nov 21, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiaohua ZhuShouzhuo Yao
Nov 3, 2017·Luminescence : the Journal of Biological and Chemical Luminescence·Soumen ChandraSumanta Kumar Sahu
Apr 20, 2021·Colloids and Surfaces. B, Biointerfaces·Legha AnsariMohammad Amjadi
Jun 17, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Xucan YuanZhili Xiong

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