Abstract
The abnormal concentrations of both biothiols and pH in lysosomes are seriously related to many major diseases, such as Parkinson's and Alzheimer's diseases. Up to now, there are few reports that clearly illustrate the relationship between lysosomal pH and biothiols via fluorescence assay. Herein, novel carbon dots (Scy-CDs) are prepared with good water dispersibility and excellent photostability, and a large Stokes shift of 106 nm is exhibited under an excitation wavelength of 450 nm. The remarkable pH-dependent behavior of Scy-CDs is presented with the fluorescence quenching based on the donor-excited photoinduced electron transfer (d-PET) process. The pKa value is 5.30, which is in good agreement with the range of the normal and abnormal lysosomal pH. Upon the addition of cysteine (Cys) or homocysteine (Hcy), the d-PET process is effectively inhibited with fluorescence recovery totally. The significant co-localization of Scy-CDs with Lyso-Tracker Deep Red in HEp-2 cells and the Pearson correlation coefficient 0.88 strongly suggest that the Scy-CDs can target lysosomal pH and Cys/Hcy in living cells.
References
Mar 2, 1990·Neuroscience Letters·M T HeafieldS G Sturman
Aug 17, 2002·Organic Letters·Bilge Turfan, Engin U Akkaya
Sep 22, 2005·Nature Reviews. Cancer·Rosa A CardoneStephan J Reshkin
Oct 22, 2008·Chemical Communications : Chem Comm·Rosa ArrigoDang Sheng Su
Dec 10, 2009·Nature Reviews. Molecular Cell Biology·Joseph R CaseyJohn Orlowski
Jun 8, 2010·Nature·Li YuMichael J Lenardo
Apr 12, 2011·The Journal of Organic Chemistry·Yuting ChenJianzhuang Jiang
Nov 30, 2012·Chemical Communications : Chem Comm·Changmin YuShuizhu Wu
Jul 3, 2013·Organic Letters·Dhiraj P MuraleDavid G Churchill
Dec 26, 2013·Bioorganic & Medicinal Chemistry Letters·Hong-Shui LvBao-Xiang Zhao
Jan 21, 2014·Acta Biomaterialia·Huanli SunZhiyuan Zhong
Jun 5, 2014·Organic & Biomolecular Chemistry·Qingxin HanWeisheng Liu
Sep 25, 2014·Nanoscale·Mani VedamalaiHuan-Tsung Chang
Sep 26, 2014·The Journal of Biological Chemistry·Dale R BalceRobin M Yates
Apr 3, 2015·Angewandte Chemie·Kai JiangHengwei Lin
Jan 14, 2016·Autophagy·Mathieu BourdenxBenjamin Dehay
Jun 24, 2016·Analytical Chemistry·Jingfang ShangguanJin Huang
Aug 9, 2017·ACS Applied Materials & Interfaces·Luling WuNengqin Jia
Mar 28, 2018·Nanoscale·Yanfeng LiuXudong Jia
Jun 28, 2018·Nanoscale·Shuang EJian-Hua Wang
Jul 25, 2018·Nanoscale·Qian Qian ZhangCheng Zhi Huang
Nov 8, 2018·Mikrochimica Acta·Wei ZhangXu-Dong Wang
Apr 18, 2019·Nanoscale·Haifang LiuYuehe Lin
Jun 4, 2019·Small·Xiaoxue YeZhihong Liu
Jan 3, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shuo GuoZhaohui Li
Citations
Oct 16, 2020·The Analyst·Dan ChangYongxing Yang
Jun 14, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Yanxia XiaoXiaojuan Gong
Oct 2, 2021·Inorganic Chemistry·Hanqing ZhaoLongshan Zhao
Oct 29, 2021·Journal of Biomedical Nanotechnology·Jiangbo FanYongzhen Yang