Carbon ion beam combined with cisplatin effectively disrupts triple negative breast cancer stem-like cells in vitro

Molecular Cancer
Sei SaiMitsuhiro Hayashi

Abstract

Although a relatively small proportion of all breast cancer (BC), triple negative (TN) BC is responsible for a relatively large proportion of BC deaths because of its worse clinical outcome. To investigate whether a carbon ion beam alone or in combination with cisplatin (CDDP) has a beneficial effect compared to X-rays, we target triple negative (TN) breast cancer stem-like cells (CSCs). Human breast CSCs sorted from MDA-MB-231 and MDA-MB-453 cells were treated with a carbon ion beam or X-ray irradiation alone or in combination with CDDP, and then colony, spheroid and tumor formation assays, RT-PCR Array analysis, and immunofluorescence γH2AX foci assay were performed. The colony, spheroid formation, and tumorigenicity assays confirmed that CD44+/CD24- and ESA+/CD24- cells have CSC properties in MDA-MB-231 and MDA-MB-453 cells, respectively. The proportion of CSCs was more enriched after CDDP combination with either X-ray or carbon ion beam, however carbon ion beam combined with CDDP significantly suppressed colony and spheroid formation and more significantly inhibited cell cycle progression (sub-G1 arrest) compared to X-ray combined with CDDP or carbon ion beam alone. RT-PCR Array analysis showed that carbon ion beam combined...Continue Reading

References

Mar 4, 1997·International Journal of Cancer. Journal International Du Cancer·B KöberleJ R Masters
Aug 5, 2000·International Journal of Radiation Oncology, Biology, Physics·M SuzukiK Ando
Jan 10, 2003·Seminars in Radiation Oncology·Theodore S LawrenceCornelius McGinn
Mar 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Muhammad Al-HajjMichael F Clarke
May 12, 2005·Molecular Cancer Research : MCR·Heather J BoeckmanJohn J Turchi
Aug 26, 2006·Stem Cells·David Dingli, Franziska Michor
Dec 13, 2006·Cancer Cell·Dolores HambardzumyanEric C Holland
Dec 21, 2006·Journal of the National Cancer Institute·Tiffany M PhillipsFrank Pajonk
Mar 14, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Daniela Schulz-Ertner, Hirohiko Tsujii
Oct 3, 2007·Cancer Research·Jeremy N Rich
Jun 24, 2009·International Journal of Radiation Oncology, Biology, Physics·Irene L IbañezHebe Durán
Aug 1, 2009·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Florian HeitzAndreas du Bois
Jan 27, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Daniel P SilverJudy E Garber
Feb 18, 2010·International Journal of Radiation Oncology, Biology, Physics·Bisrat G DebebWendy A Woodward
Oct 4, 2011·The Journal of Clinical Investigation·Kornelia Polyak
Oct 4, 2011·The Journal of Clinical Investigation·Michaela J Higgins, José Baselga
Nov 15, 2011·Breast Cancer Research : BCR·Andrew E PlaceKornelia Polyak
Dec 7, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Adam D StegCharles N Landen
Jan 10, 2012·The Lancet Oncology·Sunil Badve, Harikrishna Nakshatri
Jan 13, 2012·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Feb 4, 2012·The Lancet Oncology·Louis VermeulenJan Paul Medema
Feb 11, 2012·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Jun-Etsu MizoeUNKNOWN Organizing Committee for the Working Group for Head Neck Cancer
Mar 29, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Otto Metzger-FilhoMartine Piccart-Gebhart
May 1, 2012·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Matthew P AblettRobert B Clarke
May 5, 2012·The Lancet Oncology·Diana Pignalosa, Marco Durante
Jan 1, 2011·Frontiers in Oncology·Shirin SioshansiDavid E Wazer
Jul 17, 2012·Japanese Journal of Clinical Oncology·Hirohiko Tsujii, Tadashi Kamada
Sep 29, 2012·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Kazuhiko OonishiTadashi Kamada
Oct 9, 2012·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Nirmala Bhoo-PathyHelena M Verkooijen
Jan 3, 2013·International Journal of Cancer. Journal International Du Cancer·Neill PataniMitch Dowsett
Jan 30, 2013·Cancer Biology & Therapy·Xiaoyan NingZhaoshen Li
Mar 16, 2013·Japanese Journal of Clinical Oncology·Kota KatanodaHiroshi Nishimoto
Mar 29, 2013·Cancer Research·Kristen N StevensFergus J Couch
Jun 14, 2013·Frontiers in Oncology·Praseetha PrabhakaranLuis Filgueira
Jul 9, 2013·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Claudia PeitzschAnna Dubrovska
Aug 14, 2013·Cancer·Keiji MatsumotoUNKNOWN Working Group for Bone and Soft Tissue Sarcomas
Oct 12, 2013·The Journal of Pathology·Brian D Lehmann, Jennifer A Pietenpol

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Citations

May 10, 2017·Journal of Radiation Research·Seong-Joon ParkAkihisa Takahashi
Oct 21, 2018·Journal of Cancer Research and Clinical Oncology·Mahdi AbbasianDavid A Lightfoot
Apr 3, 2020·Journal of Radiation Research·Qiuning ZhangXiaohu Wang
Dec 18, 2018·International Journal of Particle Therapy·Sally A Amundson
Apr 23, 2020·Breast Cancer Research and Treatment·Timothy D MalouffLaura A Vallow
Jun 6, 2018·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·H GhaffariH Abdollahi
Oct 22, 2020·Cancers·Walter Tinganelli, Marco Durante
Feb 7, 2018·Clinical Oncology : a Journal of the Royal College of Radiologists·O MohamadM Durante
Jul 28, 2020·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Guillaume VaresYannick Saintigny
May 25, 2021·Frontiers in Oncology·Wanrong LuoNing-Ang Liu

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Methods Mentioned

BETA
X-ray
Profiler
PCR
flow cytometry
flow

Software Mentioned

WinRoof
RT 2 Profiler PCR Array
CellQuest
StatView

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