Carbon isotope fractionation of amino acids in fish muscle reflects biosynthesis and isotopic routing from dietary protein

The Journal of Animal Ecology
Kelton W McMahonSimon R Thorrold

Abstract

1. Analysis of stable carbon isotopes is a valuable tool for studies of diet, habitat use and migration. However, significant variability in the degree of trophic fractionation (Delta(13)C(C-D)) between consumer (C) and diet (D) has highlighted our lack of understanding of the biochemical and physiological underpinnings of stable isotope ratios in tissues. 2. An opportunity now exists to increase the specificity of dietary studies by analyzing the delta(13)C values of amino acids (AAs). Common mummichogs (Fundulus heteroclitus, Linnaeus 1766) were reared on four isotopically distinct diets to examine individual AA Delta(13)C(C-D) variability in fish muscle. 3. Modest bulk tissue Delta(13)C(C-D) values reflected relatively large trophic fractionation for many non-essential AAs and little to no fractionation for all essential AAs. 4. Essential AA delta(13)C values were not significantly different between diet and consumer (Delta(13)C(C-D) = 0.0 +/- 0.4 per thousand), making them ideal tracers of carbon sources at the base of the food web. Stable isotope analysis of muscle essential AAs provides a promising tool for dietary reconstruction and identifying baseline delta(13)C values to track animal movement through isotopically dist...Continue Reading

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Citations

Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Kelton W McMahonSimon R Thorrold
Nov 5, 2011·Biological Reviews of the Cambridge Philosophical Society·Craig A LaymanStuart Bearhop
Oct 3, 2013·PloS One·Shawn A SteffanVincent P Jones
Sep 24, 2015·Environmental Entomology·Paul A AyayeeKelli Hoover
Nov 28, 2015·Science·Kelton W McMahonThomas P Guilderson
May 29, 2012·Rapid Communications in Mass Spectrometry : RCM·Thomas LarsenDiane M O'Brien
Jul 19, 2012·Journal of Fish Biology·C N TruemanM R Palmer
Nov 29, 2013·Rapid Communications in Mass Spectrometry : RCM·Robert G WalshChristopher T Yarnes
Nov 2, 2014·Ecology and Evolution·Yoshito ChikaraishiNaohiko Ohkouchi
Jun 7, 2015·Annual Review of Nutrition·Diane M O'Brien
Apr 22, 2015·Isotopes in Environmental and Health Studies·Hanns-Ludwig SchmidtRoland A Werner
Dec 5, 2018·Rapid Communications in Mass Spectrometry : RCM·Tomasz KuderFaina Gelman
Sep 24, 2015·Rapid Communications in Mass Spectrometry : RCM·Fany SardenneNathalie Bodin
Dec 21, 2019·Journal of Fish Biology·Clark MorganJames Gelsleichter
May 31, 2018·Proceedings of the National Academy of Sciences of the United States of America·Manuel KleinerMarc Strous
May 13, 2020·Oecologia·Oliver N Shipley, Philip Matich
Jun 27, 2018·Scientific Reports·Enrique González-OrtegónLuis Giménez
Oct 17, 2019·PloS One·Mattia SaccòKliti Grice
Sep 25, 2020·Ecology·Clare L WilkinsonDarren C J Yeo

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