Carbon migration and metagenomic characteristics during anaerobic digestion of rice straw

Biotechnology for Biofuels
Dadi ChenJie Liu

Abstract

Considerable interest has been expressed in the development of anaerobic digestion (AD) of straw to solve the environmental problems caused by the dumping and burning of straw and to generate clean energy. However, the poor biodegradability of straw and the low efficiency of energy generation achieved during its AD are problematic. Studying the parameter changes involved in the process of AD is helpful for clarifying its micro-mechanisms and providing a theoretical basis for improving its efficiency. Currently, most research into process parameters has focused on gas production, methane content, pH, and volatile fatty acid (VFA) content; limited research has focused on carbon migration and functional gene changes during the AD of straw. Carbon migration and changes in metagenomic characteristics during the AD of rice straw (RS) were investigated. Accumulated biogas production was 388.43 mL/g VS. Carbon in RS was consumed, and the amount of carbon decreased from 76.28 to 36.83 g (conversion rate 51.72%). The degree of hydrolysis rapidly increased during the first 5 days, and a large amount of carbon accumulated in the liquid phase before migrating into the gas phase. By the end of AD, the amount of carbon in the liquid and gas p...Continue Reading

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Methods Mentioned

BETA
PCA
electrophoresis
DNA-Seq
PCR

Software Mentioned

ggplot2
MetaGene
HIT
BWA
MEGAHIT
ipath
CD
R
STAMP ( Statistical Analysis of Metagenomic Profiles )
SOAAPaligner

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