PMID: 2106505Mar 1, 1990Paper

Carbon monoxide dehydrogenase inhibitor in cell extracts of Pseudomonas carboxydovorans

Journal of Bacteriology
Y S DoY M Kim

Abstract

Extracts of heterotrophically grown cells of Pseudomonas carboxydovorans were found to contain an inhibitor of carbon monoxide dehydrogenase (CO-DH). The inhibitor activity was not detected in CO-autotrophically grown cells. The inhibitor was extremely stable to heat treatment based on the extent of inhibition of CO-DH activity. The extent of inhibition was proportional to the amount of cell extract added to the reaction mixture. The inhibition was independent of a prior incubation period of the extracts with CO-DH. The inhibitor was precipitable with ammonium sulfate, phenol, and trichloroacetic acid. It was passed through benzoylated dialysis tubing and Amicon ultrafiltration membrane YM2. Denaturing and nondenturing polyacrylamide gel electrophoresis of CO-DH inactivated by inhibitor revealed that the mobilities of native enzyme and subunits were identical to those of active CO-DH. The inhibitor-treated CO-DH retained its original antigenic sites and exhibited enzyme activity upon activity staining. The CO-DH inhibitor of P. carboxydovorans was also active on CO-DHs from Pseudomonas carboxydohydrogena, Acinetobacter sp. strain JC1, and Pseudomonas carboxydoflava.

References

Jan 1, 1983·International Review of Cytology·Y M Kim, G D Hegeman
Jan 1, 1983·Annual Review of Microbiology·O Meyer, H G Schlegel
Jan 1, 1980·Annual Review of Biochemistry·M Laskowski, I Kato
Dec 1, 1977·Microbial Ecology·G A Zavarzin, A N Nozhevnikova

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Citations

Jan 27, 2012·Antonie van Leeuwenhoek·Young Min Kim, Sae Woong Park
Dec 18, 2002·Journal of Bacteriology·Sae W ParkYoung M Kim
Aug 21, 2007·Biochemical and Biophysical Research Communications·Sae Woong ParkYoung Min Kim

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