Carbon nanosphere-based fluorescence aptasensor for targeted detection of breast cancer cell MCF-7

Talanta
Dandan YangZhifei Wang

Abstract

In this work, carbon nanosphere (CNS)-based fluorescence "turn off/on" aptasensor was developed for targeted detection of breast cancer cell MCF-7 by conjugation with FAM (a dye)-labeled mucin1 (MUC1) aptamer P0 (P0-FAM), which can recognize MUC1 protein overexpressed on the surface of MCF-7. Different from other carbon based fluorescence quenching materials, CNSs prepared by the carbonization of glucose not only have the high fluorescence quenching efficiency (98.8%), but also possess negligible cytotoxicity (in the concentration range of 0-1 mg/mL, which is 10 times higher than that of traditional carbon nanotubes or graphene oxide (0-100 µg/mL)). As for the detection of the mimic of the tumor antigen MUC1, the resulting fluorescence intensity increases nearly linearly in the range of 0-6 μM with the limit of detection (LOD) of 25 nM.

Citations

Apr 21, 2021·Expert Review of Molecular Diagnostics·Rajesh AhirwarSaroj Kumar
Jul 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Neda ShahbaziSeyed Morteza Naghib
Mar 30, 2019·ACS Applied Materials & Interfaces·Yuxi ZhanYunfeng Lin

❮ Previous
Next ❯

Related Concepts