Carbon Nanotube and Asbestos Exposures Induce Overlapping but Distinct Profiles of Lung Pathology in Non-Swiss Albino CF-1 Mice

Toxicologic Pathology
Evan A FrankJagjit S Yadav

Abstract

Carbon nanotubes (CNTs) are emerging as important occupational and environmental toxicants owing to their increasing prevalence and potential to be inhaled as airborne particles. CNTs are a concern because of their similarities to asbestos, which include fibrous morphology, high aspect ratio, and biopersistence. Limitations in research models have made it difficult to experimentally ascertain the risk of CNT exposures to humans and whether these may lead to lung diseases classically associated with asbestos, such as mesothelioma and fibrosis. In this study, we sought to comprehensively compare profiles of lung pathology in mice following repeated exposures to multiwall CNTs or crocidolite asbestos (CA). We show that both exposures resulted in granulomatous inflammation and increased interstitial collagen; CA exposures caused predominantly bronchoalveolar hyperplasia, whereas CNT exposures caused alveolar hyperplasia of type II pneumocytes (T2Ps). T2Ps isolated from CNT-exposed lungs were found to have upregulated proinflammatory genes, including interleukin 1ß (IL-1ß), in contrast to those from CA exposed. Immunostaining in tissue showed that while both toxicants increased IL-1ß protein expression in lung cells, T2P-specific IL...Continue Reading

References

May 1, 1978·British Journal of Cancer·J M DavisA P Middleton
Feb 1, 1992·American Journal of Respiratory Cell and Molecular Biology·K C StoneJ D Crapo
Aug 1, 1990·Environmental Health Perspectives·M Lippmann
Jun 1, 1987·The Journal of Pathology·I Y Adamson, D H Bowden
Mar 1, 1974·British Journal of Cancer·J C WagnerV Timbrell
Jan 1, 1983·The American Review of Respiratory Disease·D J SchrierS H Phan
Feb 1, 1995·Regulatory Toxicology and Pharmacology : RTP·G Oberdörster
Mar 1, 1995·American Journal of Respiratory and Critical Care Medicine·C A PopeC W Heath
Feb 1, 1996·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·T W HesterbergP Thevenaz
May 3, 1997·Lancet·G R Wagner
Jul 13, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·R WangB D Uhal
Feb 15, 2001·Occupational and Environmental Medicine·K DonaldsonW MacNee
Nov 1, 2001·Respiratory Research·H Fehrenbach
Apr 23, 2003·Free Radical Biology & Medicine·Arti ShuklaBrooke T Mossman
Jul 15, 2003·Journal of Toxicology and Environmental Health. Part a·G V S RaoAnn F Hubbs
Feb 28, 2008·The Journal of Toxicological Sciences·Atsuya TakagiJun Kanno
Jun 24, 2008·Inhalation Toxicology·Jeong Hee HanIl Je Yu
Jul 29, 2008·Toxicology and Applied Pharmacology·Seishiro HiranoAkiko Furuyama
Jul 29, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·A A ShvedovaV E Kagan
Sep 13, 2008·American Journal of Respiratory Cell and Molecular Biology·Jessica P Ryman-RasmussenJames C Bonner
Mar 23, 2011·Toxicologic Pathology·Ann F HubbsLinda M Sargent
Jul 26, 2011·Particle and Fibre Toxicology·Robert R MercerDale W Porter
Nov 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Hirotaka NagaiShinya Toyokuni
Jan 19, 2012·Journal of Toxicology and Environmental Health. Part a·J M CyphertS H Gavett
Feb 2, 2013·Science·Michael F L De VolderA John Hart
May 8, 2013·Environmental Health Perspectives·James C BonnerKent E Pinkerton
Jan 11, 2014·Particle and Fibre Toxicology·Linda M SargentSteven H Reynolds

❮ Previous
Next ❯

Methods Mentioned

BETA
transmission electron microscopy
Flow Cytometry
Assay

Software Mentioned

Zeiss Zen lite 2011
Primer BLAST
Aperio ImageScope
ImageJ
Adobe Photoshop CS2

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.