Carbon nanotube-mediated antibody-free suspension array for determination of typical endocrine-disrupting chemicals

Mikrochimica Acta
Nan LiuYiping Chen

Abstract

A carbon nanotube (CNT)-mediated antibody-free suspension array (CASA) by integration of functionalized CNTs and aptamer (Apt) into xMAP® technology for simultaneous determination of typical endocrine-disrupting chemicals (EDCs) was developed . The interaction between CNTs and Apt acts as an effective and straightforward signal recognition, transformation, and amplification strategy. The amino-functionalized CNTs are covalently modified on the carboxyl-functionalized magnetic bead (MB) and further physically bridging with biotinylated Apt. CNTs on the surface of MBs not only increase the amount of Apt for target binding and signal amplification but also maintain the biological activity of Apt. After magnetic separation, the encoded MB address was distinguished and the concentration of the target in the liquid was negatively correlated with median fluorescence intensity. A series of environmental water samples were analysed by CASA, traditional immuno-SA, and competitive inhibition enzyme-linked immunosorbent assay for validation. The results obtained using CASA well matched for the multiplexed detection of various targets with dynamic concentration range from 6.40 × 10-5 to 4.00 μg L-1 within 1 h. The method also confirmed good...Continue Reading

References

Sep 12, 1996·The New England Journal of Medicine·J L Jacobson, S W Jacobson
Apr 15, 2003·Nature Materials·Ming ZhengNancy G Tassi
Apr 7, 2005·Environmental Health Perspectives·Russ HauserAntonia M Calafat
Jun 9, 2009·Endocrine Reviews·Evanthia Diamanti-KandarakisAndrea C Gore
Dec 23, 2011·Environmental Health Perspectives·Megan E McAuliffeMelissa J Perry
Feb 4, 2012·Environmental Science & Technology·Larry B BarberJeffery H Writer
Mar 21, 2013·Chemical Reviews·Weihong TanJianhui Jiang
Oct 20, 2015·Bioanalysis·Yunting ZhuDafang Zhong
Nov 19, 2015·ACS Nano·Jitendra N TiwariKwang S Kim
Jan 28, 2016·Chemical Communications : Chem Comm·Huan WangYuanjin Zhao
Feb 3, 2016·Reviews in Endocrine & Metabolic Disorders·Dominik Rachoń
Jan 15, 2017·Current Opinion in Biotechnology·Van-Thuan NguyenMan Bock Gu
Apr 22, 2017·Angewandte Chemie·Chang LuJuewen Liu
Aug 24, 2017·Angewandte Chemie·Ying JiangWeihong Tan
Dec 7, 2017·Physical Chemistry Chemical Physics : PCCP·Ranran TianJingyuan Li

❮ Previous
Next ❯

Citations

Feb 23, 2021·Critical Reviews in Analytical Chemistry·Xue-Xia JiaZhen-Chuan Fan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Nature Medicine
Alan Dove
Pediatric Emergency Care
A V Levin
Environmental Toxicology and Chemistry
Junhoe JeongIn Seok Hong
© 2021 Meta ULC. All rights reserved