Abstract
Coagulation monitoring is essential for perioperative care and thrombosis treatment. However, existing assays for coagulation monitoring have limitations such as a large footprint and complex setup. In this work, we developed a miniaturized device for point-of-care blood coagulation testing by measuring dynamic clot retraction force development during blood clotting. In this device, a blood drop was localized between a protrusion and a flexible force-sensing beam to measure clot retraction force. The beam was featured with micropillar arrays to assist the deposition of carbon nanotube films, which served as a strain sensor to achieve label-free electrical readout of clot retraction force in real time. We characterized mechanical and electrical properties of the force-sensing beam and optimized its design. We further demonstrated that this blood coagulation monitoring device could obtain results that were consistent with those using an imaging method and that the device was capable of differentiating blood samples with different coagulation profiles. Owing to its low fabrication cost, small size, and low consumption of blood samples, the blood coagulation testing device using carbon nanotube strain sensors holds great potential ...Continue Reading
References
Mar 30, 2001·The Journal of Biological Chemistry·Y J ChuangS T Olson
Feb 20, 2003·Thrombosis Research·Marcus E CarrPhilip E Greilich
Jul 28, 2004·British Journal of Anaesthesia·A M Mahdy, N R Webster
Feb 1, 2005·Biosensors & Bioelectronics·Libby G PuckettLeonidas G Bachas
Apr 19, 2008·Anesthesia and Analgesia·Michael T Ganter, Christoph K Hofer
Apr 18, 2009·Anesthesia and Analgesia·Kenichi A TanakaJerrold H Levy
Oct 29, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Francesco ViolaWilliam F Walker
Dec 17, 2009·Analytical Chemistry·Lothar MüllerFrank K Gehring
Apr 2, 2010·Lab on a Chip·Xin M LiangNathan J Sniadecki
Dec 7, 2010·Nature Materials·Wilbur A LamDaniel A Fletcher
Dec 15, 2010·Lab on a Chip·Vijaya SunkaraYoon-Kyoung Cho
Oct 6, 2011·Anesthesiology·Klaus GörlingerJürgen Peters
May 15, 2012·Journal of Cell Science·Sarah Schwarz HenriquesSarah Köster
Apr 2, 2014·Biomedical Optics Express·Markandey M TripathiSeemantini K Nadkarni
Oct 7, 2014·Trends in Biotechnology·Xiang LiJianping Fu
Oct 30, 2014·Lab on a Chip·O CakmakHakan Urey
Jun 2, 2016·Small·Zida LiJianping Fu
Nov 1, 2016·Nature Materials·David R MyersWilbur A Lam
Sep 19, 2017·IEEE Transactions on Biomedical Circuits and Systems·Debnath MajiPedram Mohseni
Nov 9, 2018·Applied Physics Letters·Zida LiJianping Fu
Citations
Jan 11, 2020·Frontiers in Bioengineering and Biotechnology·Mohammad Mohammadi AriaOzlem Yalcin
Mar 20, 2021·ACS Applied Materials & Interfaces·Qiushun ZouChongjun Jin
Jun 18, 2020·ACS Omega·Vetrivel SankarRamaprabhu Sundara
Nov 17, 2020·ACS Sensors·Lanzhu HuangZida Li