Carbonic anhydrase III in equine tissues and sera determined by a highly sensitive enzyme-immunoassay

Equine Veterinary Journal
Toshiho Nishita, H Matsushita

Abstract

A sensitive sandwich enzyme immunoassay (EIA) for measuring equine carbonic anhydrase III (CA-III) was established using a microplate as a solid-phase and peroxidase as a labelling enzyme. The assay can detect concentrations as low as 5 ng/ml using 20 microliters of sample sera. Within-run coefficients of variation obtained using standard equine CA-III were less than 5 per cent. CA-III levels in equine serum ranged from 5 to 50 ng/ml (n = 370), and apparently abnormal levels of CA-III from 100 to 1900 ng/ml (n = 27) were observed. The concentrations of immunoreactive CA-III in the extracts of various equine tissues were also determined; it was present at high concentrations in skeletal muscle and liver and to a much lesser extent in the thymus. Other tissues contained much smaller amounts.

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Citations

Jan 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·T NishitaK Kobune
Sep 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·T NishitaM Asari
Nov 29, 2011·Acta Veterinaria Scandinavica·Toshiho NishitaKazuyosi Arishima
Feb 15, 2002·American Journal of Veterinary Research·Toshiho NishitaMasao Asari
Feb 13, 2013·Journal of Animal Physiology and Animal Nutrition·T NishitaK Arishima
Apr 20, 2000·American Journal of Veterinary Research·T NishitaM Asari
Jun 1, 1990·Anatomia, histologia, embryologia·T NishitaM Asari

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