PMID: 2510818Aug 22, 1989Paper

Carbonyl sulfide inhibition of CO dehydrogenase from Rhodospirillum rubrum

Biochemistry
M R HymanP W Ludden

Abstract

Carbonyl sulfide (COS) has been investigated as a rapid-equilibrium inhibitor of CO oxidation by the CO dehydrogenase purified from Rhodospirillum rubrum. The kinetic evidence suggests that the inhibition by COS is largely competitive versus CO (Ki = 2.3 microM) and uncompetitive versus methylviologen as electron acceptor (Ki = 15.8 microM). The data are compatible with a ping-pong mechanism for CO oxidation and COS inhibition. Unlike the substrate CO, COS does not reduce the iron-sulfur centers of dye-oxidized CO dehydrogenase and thus is not an alternative substrate for the enzyme. However, like CO, COS is capable of protecting CO dehydrogenase from slow-binding inhibition by cyanide. A true binding constant (KD) of 2.2 microM for COS has been derived on the basis of the saturable nature of COS protection against cyanide inhibition. The ability of CO, CO2, COS, and related CO/CO2 analogues to reverse cyanide inhibition of CO dehydrogenase is also demonstrated. The kinetic results are interpreted in terms of two binding sites for CO on CO dehydrogenase from R. rubrum.

References

Oct 12, 1979·Biochemical and Biophysical Research Communications·C P Chengelis, R A Neal
Nov 1, 1978·Journal of Bacteriology·G B Diekert, R K Thauer
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D BonamP W Ludden
Sep 15, 1983·Biochemical and Biophysical Research Communications·S W RagsdaleD V DerVartanian
Aug 1, 1984·Journal of Bacteriology·D BonamP W Ludden
Jul 1, 1980·Archives of Biochemistry and Biophysics·W A Laing, J T Christeller

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Citations

Feb 11, 2010·Applied Microbiology and Biotechnology·Luis A Sayavedra-SotoMark E Dolan
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·S W Ragsdale
Oct 21, 2006·Biotechnology and Bioengineering·Young S DoAlan A DiSpirito
Jun 29, 2018·Frontiers in Microbiology·Florian OswaldAnke Neumann
Jul 18, 2001·Journal of the American Chemical Society·E L MaynardP A Lindahl

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