PMID: 8943150Nov 12, 1996Paper

Carboxy-terminal sequence divergence and processing of the polyprotein antigen from Dirofilaria immitis

Molecular and Biochemical Parasitology
C B PooleL A McReynolds

Abstract

A polyprotein composed of multiple units arranged in direct tandem arrays has been identified in parasitic and free living nematodes. Analysis of previously cloned units from the Dirofilaria immitis polyprotein antigen (DiPA) indicated the units were nearly identical but here we demonstrate that they segregate into two related families. The consensus repeats, DiPA-CR1 and CR2, derived for each family are 80% identical. However, the repeats at the C-terminus of the polyprotein have diverged from DiPA-CR1 and CR2. This was shown by DNA sequence and Southern blot analysis of a 1.9 kb cDNA clone that encodes 4.4 C-terminal repeats (DiPA-TR1 through TR5). DiPA-TR3 through TR5 show 27-52% amino acid identity with the consensus repeats and 31-35% amino acid identity with one another. Metabolic labeling studies have shown that cleavage of DiPA generates a protein "ladder' from 14 to > 200 kDa. RRKR, a cleavage motif of subtilisin-like proprotein convertases, was identified as the natural cleavage site. In vitro digestion experiments with proteinase K suggest a structural model for DiPA consisting of protease resistant cores joined by protease sensitive linkers containing the RRKR site. This motif is absent between DiPA-TR3 and TR4 and ...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Apr 1, 1992·The Journal of Clinical Investigation·L X Liu, P F Weller
Aug 1, 1992·Molecular and Biochemical Parasitology·J CulpepperB Dale
Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C B PooleL A McReynolds
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jun 1, 1989·Molecular and Biochemical Parasitology·A G GrandeaL A McReynolds
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L A McReynoldsS A Williams
Jan 1, 1987·Journal of Molecular Evolution·D F Feng, R F Doolittle
Mar 1, 1988·Molecular and Biochemical Parasitology·S A WilliamsL A McReynolds
Mar 1, 1970·Journal of Molecular Biology·S B Needleman, C D Wunsch
Sep 1, 1995·Experimental Parasitology·P M AjuhT G Egwang
Apr 1, 1995·Molecular and Biochemical Parasitology·M W KennedyA Cooper
Feb 1, 1993·Molecular and Biochemical Parasitology·H J SpenceM W Kennedy
Nov 1, 1993·Parasitology Today·L A McReynoldsM E Selkirk

❮ Previous
Next ❯

Citations

Feb 12, 2000·Biochimica Et Biophysica Acta·M W Kennedy
Aug 22, 2000·Parasitology Today·M W Kennedy
Jun 24, 2003·Infection and Immunity·Hiroyuki TezukaKoichiro Fujita
Jan 30, 2014·The Journal of Parasitology·A J SassiT G Geary
Jul 12, 2011·Experimental Parasitology·Malcolm W Kennedy
Oct 6, 2014·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Gisela R FranchiniBetina Córsico

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.