PMID: 3384022May 1, 1988Paper

Carboxyfluorescein transfer across the blood-retinal barrier evaluated by quantitative fluorescence microscopy: comparison with fluorescein

Experimental Eye Research
P A Grimes

Abstract

Carboxyfluorescein levels in ocular tissues of normal rats were measured using quantitative fluorescence microscopy and compared with fluorescein levels to determine the extent to which blood-retinal barrier permeability is affected by the difference in lipid solubility of these two dyes. Retinal fluorescence intensity measurements at 2 min after i.v. dye injection were very much lower for carboxyfluorescein than for fluorescein despite similar plasma free dye concentrations. Marked leakage of dye from the optic disc into peripapillary retina was identified. At 1- and 2 hr, retinal levels of the two dyes became more similar, because fluorescein was removed from retina faster than carboxyfluorescein. After sodium-iodate-induced damage of the pigment epithelium, high levels of both dyes were evident in retina, but carboxyfluorescein was localized chiefly within extracellular space whereas fluorescein also densely stained cell somata. The fluorescence intensity levels recorded, which are proportional to the total mass of dye in the tissue, were correspondingly lower for carboxyfluorescein than for fluorescein, indicating that they were markedly affected by the different distribution of the two dyes. To relate tissue fluorescence i...Continue Reading

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Citations

Jul 16, 1999·Lasers in Surgery and Medicine·S MordonJ M Devoisselle
Jun 28, 2005·Experimental Eye Research·Gary L TrickBruce A Berkowitz
May 1, 1995·Experimental Eye Research·Y Tsukahara, D M Maurice
Sep 3, 2002·Survey of Ophthalmology·David M Maurice
Apr 1, 1994·Current Eye Research·M Kondo, M Araie
Oct 16, 2019·Korean Journal of Ophthalmology : KJO·Jae Woo KimSo Hyung Lee
Apr 2, 2020·Korean Journal of Ophthalmology : KJO·Hyun Gu Kang, Jae Woo Kim

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