Carboxylated Cy5-Labeled Comb Polymers Passively Diffuse the Cell Membrane and Target Mitochondria

ACS Applied Materials & Interfaces
Ayaat M MahmoudKristian Kempe

Abstract

A detailed understanding of the cellular uptake and trafficking of nanomaterials is essential for the design of "smart" intracellular drug delivery vehicles. Typically, cellular interactions can be tailored by endowing materials with specific properties, for example, through the introduction of charges or targeting groups. In this study, water-soluble carboxylated N-acylated poly(amino ester)-based comb polymers of different degree of polymerization and side-chain modification were synthesized via a combination of spontaneous zwitterionic copolymerization and redox-initiated reversible addition-fragmentation chain-transfer polymerization and fully characterized by 1H NMR spectroscopy and size exclusion chromatography. The comb polymers showed no cell toxicity against NIH/3T3 and N27 cell lines nor hemolysis. Detailed cellular association and uptake studies by flow cytometry and confocal laser scanning microscopy (CLSM) revealed that the carboxylated polymers were capable of passively diffusing cell membranes and targeting mitochondria. The interplay of pendant carboxylic acids of the comb polymers and the Cy5-label was identified as major driving force for this behavior, which was demonstrated to be applicable in NIH/3T3 and N2...Continue Reading

References

Mar 5, 1999·Science·D C Wallace
May 30, 2001·Advanced Drug Delivery Reviews·V Weissig, V P Torchilin
Sep 13, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Silvia MuroVladimir R Muzykantov
Jul 31, 2007·Advanced Drug Delivery Reviews·Lisa M Bareford, Peter W Swaan
Aug 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Stephanie E A GrattonJoseph M DeSimone
Jan 1, 2010·Nature Reviews. Drug Discovery·Lawrence RajendranKai Simons
Jul 6, 2010·Mitochondrion·Lucia BiasuttoJiri Neuzil
Mar 15, 2011·Antioxidants & Redox Signaling·Robin A J SmithMichael P Murphy
Jun 9, 2011·Accounts of Chemical Research·Joseph Della RoccaWenbin Lin
Jun 9, 2011·Accounts of Chemical Research·Nandanan Erathodiyil, Jackie Y Ying
Nov 22, 2011·Nanotechnology·Cristiana S O PauloLino S Ferreira
Aug 16, 2012·Accounts of Chemical Research·Motao ZhuYuliang Zhao
Mar 31, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Edgar Pérez-Herrero, Alberto Fernández-Medarde
Aug 9, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anne O Oyewole, Mark A Birch-Machin
Sep 25, 2015·Chemical Communications : Chem Comm·Kristian KempeDavid M Haddleton
Oct 16, 2015·Nature·Andrew V BiankinSimon J Hollingsworth
Mar 21, 2017·Chemical Society Reviews·Ji-Ting HouJong Seung Kim
Mar 25, 2017·ACS Applied Materials & Interfaces·Joshua J GlassStephen J Kent
Apr 7, 2017·Interface Focus·Kylie YangElizabeth J New
Jan 20, 2018·Scientific Reports·Nobuyuki MorimotoFrançoise M Winnik
Aug 14, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Lutz TauhardtUlrich S Schubert

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Citations

Aug 9, 2020·Macromolecular Rapid Communications·Nicholas L FletcherKristofer J Thurecht
Mar 27, 2020·Bioconjugate Chemistry·Ziwen JiangS Thayumanavan
Apr 20, 2021·Macromolecular Rapid Communications·Antoine NiederbergerMarkus Müllner
Jul 27, 2021·Advanced Science·Cheng YuJingzhi Ma
Mar 24, 2020·ACS Sensors·Julia Ann-Therese WalkerSimon R Corrie

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