Carboxymethyl starch/alginate microspheres containing diamine oxidase for intestinal targeting

Biotechnology and Applied Biochemistry
Lindsay BlemurMircea Alexandru Mateescu

Abstract

The association of carboxymethyl starch (CMS) and alginate is proposed as a novel matrix for the entrapment of bioactive agents in microspheres affording their protection against gastrointestinal degradation. In this study, the enzyme diamine oxidase (DAO) from white pea (Lathyrus sativus) was immobilized by inclusion in microspheres formed by ionotropic gelation of CMS/alginate by complexation with Ca(2+) . The association of CMS to alginate generated a more compact structure presenting a lesser porosity, thus decreasing the access of gastric fluid inside the microspheres and preventing the loss of entrapped enzyme. Moreover, the immobilized enzyme remained active and was able to oxidize the polyamine substrates even in the presence of degrading proteases of pancreatin. The inclusion yield in terms of entrapped protein was of about 82%-95%. The DAO entrapped in calcium CMS/alginate beads retained up to 70% of its initial activity in simulated gastric fluid (pH 2.0). In simulated intestinal fluid (pH 7.2) with pancreatin, an overall retention of 65% of activity for the immobilized DAO was observed over 24 H, whereas in similar conditions the free enzyme was totally inactivated. Our project proposes the vegetal DAO as an antihis...Continue Reading

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Citations

Jan 22, 2017·Applied Biochemistry and Biotechnology·Catherine JumarieMircea Alexandru Mateescu
Jun 5, 2019·Pharmaceutics·Nassim BenyerbahMircea Alexandru Mateescu
Oct 28, 2019·Analytical and Bioanalytical Chemistry·Oriol Comas-BastéM Carmen Vidal-Carou
Jul 6, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yilin GuoBing Wang
Aug 23, 2020·Biomolecules·Oriol Comas-BastéMaría Del Carmen Vidal-Carou
Dec 11, 2020·Scientific Reports·Armelle Tchoumi NereeMircea Alexandru Mateescu
Dec 4, 2019·International Journal of Biological Macromolecules·Nguyen Thi Thanh UyenNurazreena Ahmad
Nov 7, 2018·Journal of Agricultural and Food Chemistry·Armelle Tchoumi NereeLucia Marcocci

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Methods Mentioned

BETA
scanning electron microscopy
deamination
protein determination
electrophoresis
zymography

Software Mentioned

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