Carboxymethyllysine in dermal tissues of diabetic and nondiabetic patients with chronic renal failure: relevance to glycoxidation damage

Nephron
J MengS Horiuchi

Abstract

Carboxymethyllysine (CML) is currently recognized as a major advanced glycation end product and a marker for glycoxidation. Plasma CML levels are increased in patients with chronic renal failure (CRF). However, significance and mechanism of CML accumulation in these patients are poorly understood. The objective of the present study was to analyze CML in soluble and collagen-binding fractions of the dermis to investigate CML deposition and formation and collagen damage related to CML accumulation in patients with CRF. Skin samples (among them autopsy samples) were obtained from 33 subjects: 8 nondiabetic CRF patients, 7 diabetic predialysis patients with CRF (CRF-DM), 7 hemodialysis patients, and 11 control subjects without either CRF or DM. The dermal samples were extracted sequentially by phosphate-buffered normal saline, pepsin, and collagenase. The extracts were referred to as the soluble fraction and the proteinase-extracted fraction (including pepsin-extracted and collagenase-extracted fractions). Our ELISA assay for CML in dermal collagen from predialysis patients with CRF (CRF and CRF-DM groups) demonstrated that the levels of CML in both the soluble fraction (containing soluble CML which was mainly determined by serum c...Continue Reading

Citations

Jul 23, 2008·Transplantation·Marcus BaumannUwe Heemann
Oct 19, 2010·Applied and Environmental Microbiology·Evan D PepperSteven E Finkel
Feb 1, 2019·International Journal of Methods in Psychiatric Research·Eriko TaniHeii Arai
Jun 5, 2003·Wiener klinische Wochenschrift·Walter H Hörl
Feb 13, 2018·Experimental and Therapeutic Medicine·Jianpeng LiFangling Du
Jan 19, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Fatima AlsamadOlivier Piot

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