Carboxypeptidase E down-regulation regulates transcriptional and epigenetic profiles in pancreatic cancer cell line: A network analysis.

Cancer Biomarkers : Section a of Disease Markers
Zhile BaiYong Cheng

Abstract

Pancreatic cancer is a malignant tumor and its incidence has increased in recent years. Carboxypeptidase E (CPE) is a prohormone/proneuropeptide processing enzyme that has been shown to be associated with tumor growth and invasion in various cancers including pancreatic cancer. To understand the molecular mechanism underlying the proliferative effects of CPE in cancer cells. We down-regulated CPE gene expression in PANC-1 cell, a pancreatic cell line, and investigated mRNA, miRNA, circRNA and lncRNA expression profiling in PANC-1 cells from control group and CPE knock-down group by microarray analysis. We further validated the top 14 differentially expressed circRNAs by qRT-PCR. Our results showed that CPE down-regulation caused decreased cell proliferation. The microarray data showed 107, 15, 299 and 360 differentially expressed mRNAs, miRNAs, circRNAs, and lncRNAs, respectively between control group and CPE knock-down group. Of Which, 41 mRNAs, 12 miRNAs, 133 circRNAs, and 262 lncRNAs were down-regulated; 66 mRNAs, 3 miRNAs, 166 circRNAs, and 98 lncRNAs were up-regulated. Bioinformatics analysis showed that the top significantly enriched pathways for the differentially expressed RNAs were related to cancer onset and/or progre...Continue Reading

References

Aug 12, 2003·Journal of Molecular Biology·Christine Schubert WrightFraydoon Rastinejad
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stefano VoliniaCarlo M Croce
Mar 4, 2009·Nature Cell Biology·Julia WinterSven Diederichs
Mar 10, 2012·Endocrine Reviews·Niamh X CawleyY Peng Loh
Feb 9, 2013·Nucleic Acid Therapeutics·Yolanda Sánchez, Maite Huarte
Aug 15, 2013·Cancer Letters·Saravana R K MurthyY Peng Loh
Aug 24, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Giuseppe BaroneLouis Chesler
Feb 11, 2014·American Journal of Human Genetics·Hemanth TummalaInderjeet Dokal
Feb 18, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Peifei LiJunming Guo
Apr 25, 2015·Nature Reviews. Molecular Cell Biology·Aaron Ciechanover
Jan 25, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Shiu-Feng HuangY Peng Loh
Mar 30, 2016·Pharmacology Research & Perspectives·Tsuyoshi KonoTakashi Kojima
Apr 10, 2016·Cellular Oncology (Dordrecht)·V TaucherJ Haybaeck
Dec 14, 2016·World Journal of Gastroenterology : WJG·Milena Ilic, Irena Ilic
May 19, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Dan HanXuetao Cao
Jun 18, 2017·Biochemical Society Transactions·Zhi Hao Kwok, Yvonne Tay
Jun 6, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xiangdong BaiQiang He
Aug 17, 2018·Genesis : the Journal of Genetics and Development·Tennille SibbrittNicolas Fossat
Nov 23, 2018·BMC Medicine·Huiyun ZhuMin Li
Nov 27, 2018·Cancer Biomarkers : Section a of Disease Markers·D MoschovisM Gazouli
Oct 28, 2019·Bioscience Reports·Caizhi ChenWen Zou

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Citations

Apr 26, 2021·Pharmacology & Therapeutics·Sian ChenXian Shen

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