Carboxypeptidase M and kinin B1 receptors interact to facilitate efficient b1 signaling from B2 agonists

The Journal of Biological Chemistry
Xianming ZhangRandal A Skidgel

Abstract

Kinin B1 receptor (B1R) expression is induced by injury or inflammatory mediators, and its signaling produces both beneficial and deleterious effects. Kinins cleaved from kininogen are agonists of the B2R and must be processed by a carboxypeptidase to generate B1R agonists des-Arg(9)-bradykinin or des-Arg(10)-kallidin. Carboxypeptidase M (CPM) is a membrane protein potentially well suited for this function. Here we show that CPM expression is required to generate a B1R-dependent increase in [Ca(2+)](i) in cells stimulated with B2R agonists kallidin or bradykinin. CPM and the B1R interact on the cell membrane, as shown by co-immunoprecipitation, cross-linking, and fluorescence resonance energy transfer analysis. CPM and B1R are also co-localized in lipid raft/caveolin-enriched membrane fractions, as determined by gradient centrifugation. Treatment of cells co-expressing CPM and B1R with methyl-beta-cyclodextrin to disrupt lipid rafts reduced the B1R-dependent increase in [Ca(2+)](i) in response to B2R agonists, whereas cholesterol treatment enhanced the response. A monoclonal antibody to the C-terminal beta-sheet domain of CPM reduced the B1R response to B2R agonists without inhibiting CPM. Cells expressing a novel fusion protei...Continue Reading

References

Aug 1, 1988·Trends in Pharmacological Sciences·R A Skidgel
Jul 21, 1995·The Journal of Biological Chemistry·G B McGwire, R A Skidgel
Jan 1, 1995·Methods in Enzymology·F TanR A Skidgel
Jan 1, 1994·International Archives of Allergy and Immunology·T ShinoharaH Okada
Apr 1, 1994·Journal of Virology·K KurokiD Ganem
Jun 1, 1996·Immunopharmacology·M SiebeckJ C Cheronis
Feb 25, 1998·Biochemical and Biophysical Research Communications·E Schremmer-DanningerA A Roscher
Apr 29, 1998·Immunological Reviews·R A Skidgel, E G Erdös
Jan 19, 1999·Annual Review of Cell and Developmental Biology·D A Brown, E London
Jul 17, 1999·Trends in Endocrinology and Metabolism : TEM·E G ErdösB M Marcic
Sep 29, 1999·Trends in Biochemical Sciences·L D Fricker, E H Leiter
Oct 19, 1999·Molecular and Cellular Biology·E J SmartM P Lisanti
Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·S AngersM Bouvier
Jan 5, 2002·Cellular and Molecular Life Sciences : CMLS·S E Reznik, L D Fricker
Jan 24, 2002·Circulation·Costanza EmanueliPaolo Madeddu
Feb 21, 2002·Molecular Pharmacology·Thierry SabourinFrançois Marceau
Mar 7, 2002·The Journal of Biological Chemistry·Tatjana IgnjatovicErvin G Erdös
May 29, 2002·Cardiovascular Research·Ervin G Erdös
Sep 10, 2002·Trends in Biochemical Sciences·Anne Kenworthy
Sep 12, 2002·Pharmacological Reviews·Babak RazaniMichael P Lisanti
Dec 20, 2002·International Immunopharmacology·Ervin G Erdös, Peter A Deddish
Jul 1, 1962·Biochemical Pharmacology·E G ERDOS, E M SLOANE
Jul 16, 2004·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Makoto HagiwaraKazuaki Shimamoto
Oct 19, 2004·The Journal of Biological Chemistry·Jiang LiuEdward L Stuenkel
Feb 22, 2005·Journal of Lipid Research·Jennifer L Macdonald, Linda J Pike
Nov 12, 2005·The Journal of Pharmacology and Experimental Therapeutics·Sinisa StanisavljevicRandal A Skidgel
Feb 25, 2006·Biological Chemistry·João B Pesquero, Michael Bader

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Citations

Jun 11, 2010·Current Diabetes Reports·Edward P Feener
Dec 6, 2011·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Jerry G Webb
Apr 28, 2012·Pharmacology & Therapeutics·Domenico RegoliFernand Gobeil
Oct 3, 2009·The Journal of Allergy and Clinical Immunology·Fleur BossiFrancesco Tedesco
Oct 30, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Kathleen DeiterenAnne Marie Lambeir
Dec 18, 2009·Journal of Cellular and Molecular Medicine·Viktor BrovkovychRandal A Skidgel
Feb 24, 2011·Xenotransplantation·Guerard W ByrneChristopher G A McGregor
Jan 17, 2014·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Andrew S FoucheTimothy Craig
Jan 9, 2013·Expert Opinion on Therapeutic Targets·Catherine J Denis, Anne-Marie Lambeir
Oct 3, 2012·Thrombosis Research·Katrin Faye Nickel, Thomas Renné
Nov 28, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Catherine J DenisAnne-Marie Lambeir
Jun 2, 2016·The Journal of Allergy and Clinical Immunology·Zonne L M HofmanC Erik Hack
Jul 28, 2016·Clinical Reviews in Allergy & Immunology·Bruce L Zuraw, Sandra C Christiansen
Jun 15, 2013·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Kusumam JosephAllen P Kaplan
Dec 14, 2011·Laboratory Investigation; a Journal of Technical Methods and Pathology·Ioannis TsakirisBalazs Dezso
Oct 11, 2013·The Journal of Biological Chemistry·Xianming ZhangRandal A Skidgel
Aug 20, 2019·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Mona N OliveiraTamara T Lah
Jun 21, 2019·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Paola Bianchi GuimarãesJoão Bosco Pesquero
Mar 2, 2019·Inflammopharmacology·Nara L M QuintãoJoão B Calixto
Sep 4, 2020·International Journal of Molecular Sciences·Haibo YuYong Zhao
Aug 12, 2019·Translational Research : the Journal of Laboratory and Clinical Medicine·Inga Sörensen-ZenderRoland Schmitt
Jan 22, 2021·European Journal of Pharmacology·Indiara BruscoSara Marchesan Oliveira
Jul 1, 2021·Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology·Julia Hews-Girard, Marilyn Dawn Goodyear

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