Carboxypeptidase S- and carboxypeptidase Y-deficient mutants of Saccharomyces cerevisiae.

Journal of Bacteriology
D H Wolf, C Ehmann

Abstract

A new carboxypeptidase (carboxypeptidase S) was found in a Saccharomyces cerevisiae strain lacking carboxypeptidase Y (D. H. Wolf and U. Weiser, Eur. J. Biochem. 73:553-556, 1977). Mutants devoid of carboxypeptidase S activity were isolated from a mutant strain that was also deficient in carboxypeptidase Y. Four mutants were analyzed in detail and fell into one complementation group. The defect segregated 2:2 in meiotic tetrads. Gene dosage experiments indicated that the mutation might reside in the structural gene of carboxypeptidase S. The absence of both enzymes, carboxypeptidases Y and S, did not affect mitotic growth. Ascopore formation was only slightly affected by the absence of both carboxypeptidases. Protein degradation under conditions of nutrient deprivation and under sporulation conditions showed no obvious alteration in the absence of carboxypeptidases Y and S. When a proteinase B mutation, which led to the absence of proteinase B activity and resulted in the partial reduction of sporulation, was introduced into a mutant lacking both carboxypeptidases, the ability of diploid cells to sporulate was nearly completely lost. Mutants lacking both carboxypeptidases were unable to grow on the dipeptide benzyloxycarbonylgl...Continue Reading

References

Aug 1, 1976·Analytical Biochemistry·R J HansenH Holzer
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Sep 1, 1975·Journal of Bacteriology·D H Wolf, G R Fink
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G S Zubenko, E W Jones
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Aug 1, 1979·European Journal of Biochemistry·D H Wolf, C Ehmann
Feb 2, 1976·European Journal of Biochemistry·H Betz, U Weisner
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Aug 15, 1969·Molecular & General Genetics : MGG·F K ZimmermannA M ten Berge
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Apr 1, 1981·Archives of Biochemistry and Biophysics·T AchstetterD H Wolf
Jan 1, 1965·Analytical Biochemistry·C E MCDONALD, L L CHEN

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Citations

Oct 9, 1999·Yeast·M V FloresC E Voget
May 21, 2014·Cellular Logistics·Karen A HechtJeffrey L Brodsky
May 2, 1988·European Journal of Biochemistry·H H HirschD H Wolf
Mar 2, 1987·European Journal of Biochemistry·N García-AlvarezD H Wolf
Jan 18, 2007·FEMS Microbiology Letters·Zahuiti Hernández-MontañezLourdes Villa-Tanaca
Apr 23, 1991·European Journal of Biochemistry·D O SpormannD H Wolf
Dec 1, 1981·European Journal of Biochemistry·B Mechler, D H Wolf
Mar 10, 1995·Molecular & General Genetics : MGG·J Bordallo, P Suárez-Rendueles
Nov 30, 1982·Biochemical and Biophysical Research Communications·T AchstetterD H Wolf
Oct 1, 1983·Archives of Biochemistry and Biophysics·T AchstetterD H Wolf
Aug 1, 1982·Biochemical and Biophysical Research Communications·B MechlerD H Wolf
Nov 15, 1983·Biochemical and Biophysical Research Communications·O EmterD H Wolf

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