Carcass mass has little influence on the structure of gravesoil microbial communities

International Journal of Legal Medicine
Sophie WeissRob Knight

Abstract

Little is known about how variables, such as carcass mass, affect the succession pattern of microbes in soils during decomposition. To investigate the effects of carcass mass on the soil microbial community, soils associated with swine (Sus scrofa domesticus) carcasses of four different masses were sampled until the 15th day of decomposition during the month of June in a pasture near Lincoln, Nebraska. Soils underneath swine of 1, 20, 40, and 50 kg masses were investigated in triplicate, as well as control sites not associated with a carcass. Soil microbial communities were characterized by sequencing the archaeal, bacterial (16S), and eukaryotic (18S) rRNA genes in soil samples. We conclude that time of decomposition was a significant influence on the microbial community, but carcass mass was not. The gravesoil associated with 1 kg mass carcasses differs most compared to the gravesoil associated with other carcass masses. We also identify the 15 most abundant bacterial and eukaryotic taxa, and discuss changes in their abundance as carcass decomposition progressed. Finally, we show significant decreases in alpha diversity for carcasses of differing mass in pre-carcass rupture (days 0, 1, 2, 4, 5, and 6 postmortem) versus post-c...Continue Reading

References

Sep 1, 1991·The American Journal of Forensic Medicine and Pathology·K A Hewadikaram, M L Goff
Jun 4, 1999·Trends in Ecology & Evolution·T Bongers, H Ferris
Jul 18, 2001·Forensic Science International : Synergy·C P Campobasso, F Introna
Oct 26, 2001·The Annals of Pharmacotherapy·C M KirkpatrickJ Vella-Brincat
Mar 28, 2002·Applied and Environmental Microbiology·Cheryl R KuskeJayne Belnap
Dec 8, 2005·Applied and Environmental Microbiology·Catherine A Lozupone, Rob Knight
Apr 6, 2006·Environmental Microbiology·Rutger de Wit, Thierry Bouvier
Nov 9, 2006·Die Naturwissenschaften·David O CarterMark Tibbett
Jun 3, 2009·Journal of Forensic Sciences·Tamsin C F Haslam, Mark Tibbett
Mar 1, 2002·FEMS Microbiology Ecology·A M IbekweD E Crowley
Mar 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Noah FiererRob Knight
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Apr 20, 2010·Forensic Science International : Synergy·David O CarterMark Tibbett
Dec 28, 2010·Trends in Ecology & Evolution·Jeffery K TomberlinRachel M Mohr
Feb 1, 2011·Forensic Science International : Synergy·Ashley SpickaDavid O Carter
Jan 13, 2012·The ISME Journal·Qun MaThomas K Wood
Nov 30, 2012·Nucleic Acids Research·Christian QuastFrank Oliver Glöckner
Apr 5, 2013·Journal of Forensic Sciences·Géraldine MaujeanDaniel Malicier
Jun 12, 2013·International Journal of Legal Medicine·Jennifer L PechalJeffery K Tomberlin
Jul 5, 2013·Journal of Forensic Sciences·Jessica MeyerDavid O Carter
Jul 31, 2013·Forensic Science International : Synergy·A SutherlandP J Becker
Oct 1, 2013·Nature Methods·Joseph PaulsonMihai Pop
Nov 23, 2013·PloS One·Jennifer L PechalM Eric Benbow
Feb 4, 2014·International Journal of Legal Medicine·Szymon MatuszewskiMichał Szafałowicz
Jun 8, 2014·Applied and Environmental Microbiology·Christian L LauberRob Knight

Citations

Jan 11, 2016·Microbial Ecology·Sheree J FinleyGulnaz T Javan
Nov 9, 2018·Journal of Forensic Sciences·Whitney A KodamaChristopher B Happy
Oct 2, 2020·Applied Microbiology and Biotechnology·Hirak Ranjan Dash, Surajit Das
Sep 28, 2017·BMC Microbiology·Karoline ValsethThomas H A Haverkamp
Jun 6, 2018·The ISME Journal·David PreiswerkDieter Ebert

Related Concepts

Metazoa
Filamentous fungus
Livor Mortis
RNA, Bacterial
RNA, Ribosomal, 16S
RNA, Ribosomal, 18S
Soil Microbiology
Family suidae
Sequence Determinations, RNA
Genes

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

The Tendon Seed Network

Tendons are rich in the extracellular matrix and are abundant throughout the body providing essential roles including structure and mobility. The transcriptome of tendons is being compiled to understand the micro-anatomical functioning of tendons. Discover the latest research pertaining to the Tendon Seed Network here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Incretins

Incretins are metabolic hormones that stimulate a decrease in glucose levels in the blood and they have been implicated in glycemic regulation in the remission phase of type 1 diabetes. Here is the latest research.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Long COVID-19

“Long Covid-19” describes illness in patients who are reporting long-lasting effects of the SARS-CoV-19 infection, often long after they have recovered from acute Covid-19. Ongoing health issues often reported include low exercise tolerance and breathing difficulties, chronic tiredness, and mental health problems such as post-traumatic stress disorder and depression. This feed follows the latest research into Long Covid.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.