Carcinine has 4-hydroxynonenal scavenging property and neuroprotective effect in mouse retina.

Investigative Ophthalmology & Visual Science
Lea D MarchetteAnne Kasus-Jacobi

Abstract

Oxidative stress induces retinal damage and contributes to vision loss in progressive retinopathies. Carcinine (β-alanyl-histamine) is a natural imidazole-containing peptide derivative with antioxidant activity. It is predicted to scavenge 4-hydroxynonenal (4-HNE), a toxic product of lipid oxidation. The aim of this study was to confirm the 4-HNE scavenging effect and evaluate the neuroprotective effect of carcinine in mouse retina subjected to oxidative stress. HPLC coupled with mass spectrometry was used to analyze carcinine and 4-HNE-carcinine adduct. Protection of retinal proteins from modification by 4-HNE was tested by incubating carcinine with retinal protein extract and 4-HNE. Modified retinal proteins were quantified by dot-blot analysis. Mice were treated with carcinine (intravitreal injection and gavage) and exposed to bright light to induce oxidative damage in the retina. Photoreceptor degeneration was measured by histology and electroretinography. Retinal levels of retinol dehydrogenase 12 (RDH12) were measured by immunoblot analysis, after exposure to bright light and in retinal explants after exposure to 4-HNE. The ability of carcinine to form an adduct with 4-HNE, as well as to prevent and even reverse the adduc...Continue Reading

Citations

Mar 1, 2016·Cell Reports·Ratna ChaturvediHong-Sheng Li
Mar 19, 2020·International Journal of Molecular Sciences·Darcy C EngelhartSanjay K Nigam
Sep 13, 2019·Ophthalmic Genetics·Samiksha Fouzdar-JainWilliam B Rizzo
Oct 11, 2017·Current Pharmacology Reports·Sean S Davies, Linda S Zhang

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