PMID: 6988326Feb 1, 1980Paper

Carcinoembryonic antigen production by human colorectal adenocarcinoma cells in matrix-perfusion culture

In Vitro
J M QuarlesA Leibovitz

Abstract

Four human colorectal adenocarcinoma tumor cell lines, previously established and characterized in monolayer culture were grown in a matrix-perfusion culture system to determine the suitability of this technique for synthesis of carcinoembryonic antigen (CEA). Production of CEA in excess of 100,000 ng was attained from one cell line, SW 403, during 15-day growth trials. In growth trials and cell-free diffusion studies, CEA passed through membranes of 100,000-dalton molecular weight porosity but not 10,000 porosity. Using cell cultures of high, moderate, or low producers, CEA synthesis tended to reach a plateau after several days of culture and remained nearly constant as the cells attained a maintenance condition. Basic biologic characteristics of the cell lines, expressed as growth rates and CEA produced per 10(6) cells, were comparable in monolayer and perfusion culture. The high cell densities, (10(8) to 10(9) cells per ml) achieved in matrix perfusion made it possible to routinely obtain continuous high yields of CEA over an extended time period.

References

Jan 1, 1977·The Journal of Infectious Diseases·P GerhardtR C Belding
Mar 1, 1976·British Journal of Cancer·A T IchikiJ Eveleigh

❮ Previous
Next ❯

Citations

Oct 1, 1994·Biotechnology and Bioengineering·D J OhH N Chang
Jan 1, 1990·Biotechnology Advances·J M Piret, C L Cooney
Jan 1, 1985·Critical Reviews in Oncology/hematology·J E Shively, J D Beatty
Nov 16, 2002·The Journal of Physiology·Annick Guyot, John W Hanrahan
Dec 14, 2011·Molecular Carcinogenesis·Lauren C KimlinVictoria M Virador
Mar 16, 2000·Journal of Biomaterials Science. Polymer Edition·A HiguchiM Hara
Mar 1, 1985·International Journal of Cell Cloning·S E SingletaryG Blumenschein
Jan 1, 1986·International Journal of Cell Cloning·D D Von HoffL E Warfel
Mar 1, 1991·International Journal of Cell Cloning·F Baker, L Sanger
Jul 1, 1990·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·T KobayashiT Kawakubo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.