PMID: 11921188Mar 29, 2002Paper

Carcinogen-DNA adducts in human breast epithelial cells

Environmental and Molecular Mutagenesis
Katarzyna Gorlewska-RobertsFred F Kadlubar

Abstract

Diet and environmental exposures are often regarded as significant etiologic factors in human breast cancer. Chemicals that may be involved in these exposures include heterocyclic amines, aromatic amines, and polycyclic aromatic hydrocarbons, which also serve as strong mammary carcinogens in different animal models. In this study, we chose to quantify the major DNA adducts derived from one member of each of these classes of carcinogens, that is, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), 4-aminobiphenyl (ABP), and benzo[a]pyrene (B[a]P), respectively, in DNA isolated from exfoliated ductal epithelial cells in human breast milk. Milk was collected from healthy, nonsmoking mothers. The isolated DNA was digested to 3' nucleotides and subjected to (32)P-postlabeling. Adduct enrichment was achieved using Oasis Sep-Paks and the analyses were conducted by HPLC using radiometric detection. Critical to the analyses were the syntheses of bis(phosphate) standards for the C8-dG adducts of PhIP and ABP, and the N(2)-dG adduct of B[a]P, which were added to each reaction as UV markers. Of the 64 samples analyzed, adducts were found in 31 samples. Thirty samples contained detectable levels of PhIP adducts, with a mean value of 4.7...Continue Reading

References

Sep 1, 1992·Chemical Research in Toxicology·K el-Bayoumy
Jul 1, 1992·Medical Hypotheses·J J Morris, E Seifter
Jul 1, 1992·Carcinogenesis·A C Beach, R C Gupta
Jan 1, 1987·Analytical Chemistry·R OtsonL T Chung
Mar 15, 1993·Cancer·I C Henderson
Jan 1, 1996·Annual Review of Pharmacology and Toxicology·M S WolffJ Huff
Nov 1, 1996·Carcinogenesis·H S Feigelson, B E Henderson
Nov 25, 1998·Journal of the National Cancer Institute·W ZhengA R Folsom
Mar 13, 1999·International Journal of Cancer. Journal International Du Cancer·D M ParkinJ Ferlay
Nov 20, 2001·Chemical Research in Toxicology·L S DeBruinP D Josephy
Mar 29, 2002·Environmental and Molecular Mutagenesis·Patricia A ThompsonChristine B Ambrosone

❮ Previous
Next ❯

Citations

Jul 27, 2007·Breast Cancer Research and Treatment·Nalin SiriwardhanaHwa-Chain Robert Wang
Apr 10, 2010·Breast Cancer Research and Treatment·Noel PabalanHilmi Ozcelik
Oct 17, 2006·Chemical Research in Toxicology·Joseph R KurianLauren A Trepanier
Mar 16, 2007·Journal of Exposure Science & Environmental Epidemiology·Judy S LaKindMichael N Bates
Sep 16, 2008·Nutrition and Cancer·Kristie M SullivanNeal D Barnard
Dec 1, 2007·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·R Y Wang, L L Needham
Apr 23, 2005·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Glenn Talaska, Mustafa Al-Zoughool
Dec 22, 2010·Nicotine & Tobacco Research : Official Journal of the Society for Research on Nicotine and Tobacco·Liqin ZhangClifford H Watson
Sep 19, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·David W HeinMark A Doll
May 1, 2010·The Journal of Pharmacology and Experimental Therapeutics·Mark A DollDavid W Hein
Apr 12, 2005·Annual Review of Pharmacology and Toxicology·Donghak Kim, F Peter Guengerich
Feb 18, 2014·Endocrine-related Cancer·Kevin C KnowerColin D Clyne
Jan 16, 2007·Breast Cancer Research and Treatment·Carmen LillaJenny Chang-Claude
Jan 21, 2014·Chemical Research in Toxicology·Silvia BalboPeter W Villalta
Apr 10, 2004·Cancer Science·Takashi SugimuraMinako Nagao
Oct 13, 2007·Molecular Carcinogenesis·Nalin Siriwardhana, Hwa-Chain Robert Wang
Apr 19, 2016·DNA Repair·Satya NarayanRobert A Hromas
Dec 15, 2010·Biochimica Et Biophysica Acta·Run-Yue Huang, George G Chen
Jul 3, 2007·Archives of Biochemistry and Biophysics·Christine B AmbrosoneFred F Kadlubar
Oct 2, 2007·Toxicology and Applied Pharmacology·Bekim SadikovicDavid I Rodenhiser
Nov 6, 2004·Carcinogenesis·Liang ShanElizabeth G Snyderwine
Jun 13, 2017·Carcinogenesis·Adana A M LlanosChristine B Ambrosone
Aug 27, 2002·Nature Genetics·Anne-Renee Hartman, James M Ford
May 17, 2019·High-throughput·Jingshu Guo, Robert J Turesky
Sep 2, 2016·Current Protocols in Nucleic Acid Chemistry·Jingshu Guo, Robert J Turesky
Nov 2, 2016·Organic & Biomolecular Chemistry·Susanta AdhikariDebasis Das

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.