PMID: 2496751Jan 24, 1989Paper

Carcinogen-nucleic acid interactions: equilibrium binding studies of aflatoxin B1 with the oligodeoxynucleotide d(ATGCAT)2 and with plasmid pBR322 support intercalative association with the B-DNA helix

Biochemistry
S GopalakrishnanT M Harris

Abstract

Equilibrium binding of aflatoxin B1 (AFB1) to the oligodeoxynucleotide d(ATGCAT)2 was examined by using 1H NMR. AFB1 binds to double-stranded d(ATGCAT)2 with an apparent binding constant of 3.7 x 10(3) M-1. The equilibrium is rapid on the NMR time scale; the observed 1H NMR spectrum represents the population-weighted average of the chemical shifts arising from the free and bound states of the oligodeoxynucleotide and the AFB1. The spectrum of d(ATGCAT)2 exhibits exchange broadening in the presence of AFB1, manifested as decreases in apparent T2 relaxation times for the d(ATGCAT)2 base protons. Upon binding to d(ATGCAT)2, the AFB1 signals are shifted upfield, indicative of increased shielding. The adenine H2 protons are also shifted upfield in the presence of the carcinogen. Small changes in chemical shift are observed for other d(ATGCAT)2 protons. A substantial decrease in the nonselective T1 relaxation time is observed for the adenine H2 protons in the presence of AFB1. Competition binding experiments in which the competing ligands actinomycin D, ethidium bromide, and spermidine were individually added to an AFB1-d(ATGCAT)2 equilibrium mixture showed that addition of 1 equiv of actinomycin D or 4 equiv of ethidium bromide was ...Continue Reading

References

May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J M EssigmannG N Wogan
Nov 1, 1976·Biopolymers·C Giessner-PrettreP O Ts'o
Jun 14, 1969·Journal of Molecular Biology·M SuwalskyJ A Subirana
May 1, 1967·The Biochemical Journal·J I Clifford, K R Rees
May 1, 1967·Plant Physiology·H S Black, B Jirgensons
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·K F MuenchM Z Humayun
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·J M EssigmannG N Wogan
Aug 31, 1982·Biochemical and Biophysical Research Communications·C Giessner-Prettre, B Pullman
Dec 20, 1982·Nucleic Acids Research·S A WinkleT R Krugh
Sep 25, 1981·Journal of Molecular Biology·H R Drew, R E Dickerson

❮ Previous
Next ❯

Citations

Mar 29, 2002·Chemistry : a European Journal·Masanori YamadaNorio Nishi
Sep 14, 2002·Biopolymers·Indrajit Giri, Michael P Stone
May 1, 1994·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·D Kumari, S P Sinha
Jun 1, 1994·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·S Bose, S P Sinha
Sep 24, 2011·Chemical Research in Toxicology·Kathryn E PietschShana J Sturla
Sep 6, 2012·Chemical Research in Toxicology·Igor ShamovskyPeter Sjö
Apr 15, 1997·Nucleic Acids Research·W A LaMarrP C Dedon
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·W W Johnson, F P Guengerich
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Maryann E SmelaJohn M Essigmann
Jul 28, 2011·Journal of the American Chemical Society·Surajit BanerjeeMichael P Stone
Jan 18, 2011·Analytical Biochemistry·Mohammad Hossein BanitabaAli Mehdinia
Jan 6, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Martin Klvana, Urban Bren
Jun 18, 2002·Chemical Research in Toxicology·F Peter GuengerichThomas M Harris
Aug 26, 1998·Chemical Research in Toxicology·W R JonesM P Stone
Dec 19, 2003·Chemical Research in Toxicology·Hong Zang, Kent S Gates

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.