PMID: 9422099Jan 9, 1998Paper

Carcinoma-associated MUC1 detected by immunoradiometric assays

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
L NorumK Nustad

Abstract

Fifty-four anti-MUC1 antibodies submitted to the International Society for Oncodevelopmental Biology and Medicine (ISOBM) Workshop (TD-4) were evaluated in immunoradiometric assays, using sera from carcinoma patients and healthy donors. The carcinoma serum pool contained sera from 30 patients with advanced cancer (10 breast, 10 colon, and 10 ovarian). This serum pool contained 696 kU/l MUC1, 770 micrograms/l CEA, and 3,700 kU/l CA 125. The reference serum pool was obtained from 10 healthy women combined with 20 sera from pregnant women, of which half had elevated CA 125 (range 82-254 kU/l). The reference serum pool contained 13 kU/l MUC1, 2 micrograms/l CEA, and 65 kU/l CA 125. The Workshop antibodies were tested both as solid-phase antibodies and as tracer antibodies with the carcinoma serum pool. Twenty-two tracer antibodies and 38 solid-phase antibodies gave at least one combination with > 10% binding of the tracer antibody for a total of 836 combinations. These were tested further with the reference serum pool. Antibodies used as tracers could be separated into three categories: Group 1 antibodies, MF06, MF11, B27.29, MF30, and Ma552, gave mainly 'carcinoma-specific' assays in combinations with the solid-phase antibodies, i...Continue Reading

Citations

Feb 21, 2006·Biochemistry. Biokhimii︠a︡·S J Golubović, Z T Bojić-Trbojević
Jul 24, 2004·Critical Reviews in Clinical Laboratory Sciences·Stephan E BaldusFranz-Georg Hanisch
Mar 18, 2003·International Journal of Cancer. Journal International Du Cancer·Stéphanie TruantGuillemette Huet

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