Cardamonin induces G2/M arrest and apoptosis via activation of the JNK-FOXO3a pathway in breast cancer cells

Cell Biology International
Weiwei KongKaiwen Chang

Abstract

Cardamonin (CD), a naturally occurring chalcone isolated from large black cardamom, was previously reported to suppress the proliferation of breast cancer cells. However, its precise molecular anti-tumor mechanisms have not been well elucidated. In this study, we found that CD markedly inhibited the proliferation of MDA-MB 231 and MCF-7 breast cancer cells through the induction of G2/M arrest and apoptosis. Reactive oxygen species (ROS) plays a pivotal role in the inhibition of CD-induced cell proliferation. Treatment with N-acetyl-cysteine (NAC), an ROS scavenger, blocked CD-induced G2/M arrest and apoptosis in this study. Quenching of ROS by overexpression of catalase also blocked CD-induced cell cycle arrest and apoptosis. We showed that CD enhanced the expression and nuclear translocation of Forkhead box O3 (FOXO3a) via upstream c-Jun N-terminal kinase, inducing the expression of FOXO3a and its target genes, including p21, p27, and Bim. This process led to the reduction of cyclin D1 and enhancement of activated caspase-3 expression. The addition of NAC markedly reversed these effects, knockdown of FOXO3a using small interfering RNA also decreased CD-induced G2/M arrest and apoptosis. In vivo, CD efficiently suppressed the g...Continue Reading

References

Jun 1, 1976·Planta medica·C Bheemasankara RaoS Suryaprakasam
Oct 23, 2002·Molecular and Cellular Biology·Marc SchmidtRené H Medema
Oct 7, 2003·The Journal of Biological Chemistry·Andrew SuntersEric W-F Lam
Jun 8, 2004·Current Pharmaceutical Design·Shazib Pervaiz, Marie-Veronique Clement
Nov 13, 2004·The EMBO Journal·Marieke A G EssersBoudewijn M T Burgering
Jul 13, 2005·The Journal of Cell Biology·Jun SunayamaYukiko Gotoh
Nov 24, 2007·Molecular and Cellular Endocrinology·Tomohiro Nakamura, Kazuichi Sakamoto
May 30, 2009·Nature Reviews. Drug Discovery·Dunyaporn TrachoothamPeng Huang
Oct 6, 2009·Biochemical and Biophysical Research Communications·Munju ChoSangtaek Oh
Nov 16, 2012·Revista Da Sociedade Brasileira De Medicina Tropical·Maxwel Adriano AbeggMara Silveira Benfato
Aug 24, 2013·PloS One·Ying JiangAi-Guo Shen
Jan 7, 2014·Molecular and Cellular Biochemistry·Weiyan HeNan Zhang
May 13, 2014·Anti-cancer Drugs·Hao LiuZhimin He
Jul 8, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ning WuXiukun Lin
Jul 4, 2016·Biomedical Journal·Emma Louise Walton
Nov 29, 2016·Postȩpy higieny i medycyny doświadczalnej·Agata SarniakStanisława Lipińska
Jul 13, 2017·Current Medicinal Chemistry·Hande Gurer-OrhanSibel Suzen
Sep 9, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Isola A M Brown, Brian D Gulbransen
Jul 10, 2018·Toxicology Reports·Rochelle N WickramasekaraDeniz Yilmazer-Hanke
Jul 19, 2018·Oncotarget·Mildred C GonzalesShyang-Yun Pamela K Shiao
Jul 27, 2018·Molecular Cancer·Ying LiuJianxun Wang

❮ Previous
Next ❯

Citations

Dec 29, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lulu ZhangJinyong Luo
May 6, 2021·Biomolecules·Shirley JamesKuzhuvelil B Harikumar
Jun 3, 2021·Cancers·Radka MichalkovaJan Mojzis
Aug 8, 2021·Journal of Experimental & Clinical Cancer Research : CR·Li DingXinyue Zhang
Dec 7, 2021·Natural Product Research·Paloma Silva de SouzaMichelle Frazão Muzitano

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis