Cardamonin Suppresses TGF-β1-Induced Epithelial Mesenchymal Transition via Restoring Protein Phosphatase 2A Expression

Biomolecules & Therapeutics
Eun Ji KimChang Hoon Lee

Abstract

Epithelial mesenchymal transition (EMT) is the first step in metastasis and implicated in the phenotype of cancer stem cells. Therefore, understanding and controlling EMT, are essential to the prevention and cure of metastasis. In the present study, we examined, by Western blot, reverse transcription polymerase chain reaction (RT-PCR), and confocal microscopy, the effects of cardamonin (CDN) on transforming growth factor-β1 (TGF-β1)-induced EMT of A549 lung adenocarcinoma cell lines. TGF-β1 induced expression of N-cadherin and decreased expression of E-cadherin. CDN suppressed N-cadherin expression and restored E-cadherin expression. Further, TGF-β1 induced migration and invasion of A549 cancer cells, which was suppressed by CDN. TGF-β1 induced c-Jun N-terminal kinase (JNK) activation during EMT, but CDN blocked it. Protein serine/threonine phosphatase 2A (PP2A) expression in A549 cancer cells was reduced by TGF-β1 but CDN restored it. The overall data suggested that CDN suppresses TGF-β1-induced EMT via PP2A restoration, making it a potential new drug candidate that controls metastasis.

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Citations

Jan 23, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Kyoung Ah KangJin Won Hyun
Jan 16, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jianpeng WangWeicheng Yao
Mar 10, 2017·Biomolecules & Therapeutics·June Hee KangChang Hoon Lee
Mar 20, 2018·British Journal of Cancer·Ion CristóbalJesús García-Foncillas
Mar 22, 2019·Frontiers in Cell and Developmental Biology·Diana SchuhmacherEstelle Sontag
Mar 11, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Jinzhao HeBaoxue Yang

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Methods Mentioned

BETA
surgical resection
PCR
confocal microscopy

Software Mentioned

quick
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