Cardiac β-Adrenoceptor Expression Is Reduced in Zucker Diabetic Fatty Rats as Type-2 Diabetes Progresses

PloS One
James M HaleyJean N DaSilva

Abstract

Reduced cardiac β-adrenoceptor (β-AR) expression and cardiovascular dysfunction occur in models of hyperglycemia and hypoinsulinemia. Cardiac β-AR expression in type-2 diabetes models of hyperglycemia and hyperinsulinemia, remain less clear. This study investigates cardiac β-AR expression in type-2 diabetic Zucker diabetic fatty (ZDF) rats. Ex vivo biodistribution experiments with [3H]CGP12177 were performed in Zucker lean (ZL) and ZDF rats at 10 and 16 weeks of age as diabetes develops. Blood glucose, body mass, and diet consumption were measured. Western blotting of β-AR subtypes was completed in parallel. Echocardiography was performed at 10 and 16 weeks to assess systolic and diastolic function. Fasted plasma insulin, free fatty acids (FFA), leptin and fed-state insulin were also measured. At 10 weeks, myocardial [3H]CGP12177 was normal in hyperglycemic ZDF (17±4.1mM) compared to ZL, but reduced 16-25% at 16 weeks of age as diabetes and hyperglycemia (22±2.4mM) progressed. Reduced β-AR expression not apparent at 10 weeks also developed by 16 weeks of age in ZDF brown adipose tissue. In the heart, Western blotting at 10 weeks indicated normal β1-AR (98±9%), reduced β2-AR (76±10%), and elevated β3-AR (108±6). At 16 weeks, β1-...Continue Reading

References

Jan 20, 1995·Journal of Chromatography. B, Biomedical Applications·A van WaardeW Vaalburg
Aug 17, 1999·Biochemical Pharmacology·N MatsudaM Kanno
Jan 29, 2000·Naunyn-Schmiedeberg's Archives of Pharmacology·N MatsudaM Kanno
Jun 1, 2000·Metabolism: Clinical and Experimental·G J Etgen, B A Oldham
Dec 5, 2000·The Journal of Biological Chemistry·L HuangC Li
Jan 31, 2003·The New England Journal of Medicine·Peter GaedeOluf Pedersen
Feb 6, 2004·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Sabine FredersdorfJoachim Weil
Mar 14, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Susan A MarshJohn C Chatham
Jun 20, 2008·Diabetes, Obesity & Metabolism·C L OltmanM A Yorek
Apr 16, 2009·JAMA : the Journal of the American Medical Association·Lawrence H YoungUNKNOWN DIAD Investigators
Apr 25, 2009·Journal of the American College of Cardiology·Bertrand RozecChantal Gauthier
Sep 4, 2009·Disease Models & Mechanisms·Heiko Bugger, E Dale Abel
Apr 23, 2010·Journal of Hypertension·Jorge ToblliMargarita Angerosa
Jun 1, 2011·Heart Failure Reviews·Inês Falcão-Pires, Adelino F Leite-Moreira
Nov 9, 2011·Nature Reviews. Endocrinology·Lei ChenPaul Z Zimmet
Jan 19, 2012·European Journal of Heart Failure·Anneleen DanielsFrans A van Nieuwenhoven
Dec 29, 2012·The Journal of Clinical Endocrinology and Metabolism·Nora E StraznickyElisabeth A Lambert
Jan 17, 2013·Cardiovascular Diabetology·Márta SárközyTamás Csont
Jul 10, 2013·Wideochirurgia i inne techniki mało inwazyjne = Videosurgery and other miniinvasive techniques·Jodok Matthias GruenebergerSimon Küsters
Jul 12, 2013·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·James T ThackerayJean N DaSilva
Aug 31, 2013·Circulation Research·Anastasios LymperopoulosWalter J Koch

❮ Previous
Next ❯

Citations

Aug 15, 2018·The Journal of Pharmacology and Experimental Therapeutics·Abubakr MossaLysanne Campeau
Dec 2, 2020·Cells·Betul R ErdoganEbru Arioglu-Inan
Jan 9, 2021·The FEBS Journal·Kirstie A De Jong, Viacheslav O Nikolaev
Feb 21, 2021·Clinical and Experimental Pharmacology & Physiology·Ahmed SultanFrank Christopher Howarth

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA

Software Mentioned

AlphaEase FC
VisualSonics

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Cardiovascular Disorder in Diabetes

Diabetes is associated with an increased risk of cardiovascular disorders and heart failure. Discover the latest research here.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.

CV Disorders & Type 2 Diabetes

This feed focuses on the association of cardiovascular diseases in patients with type 2 diabetes.

Cardiovascular Disease Pathophysiology

Cardiovascular disease involves several different processes that contribute to the pathological mechanism, including hyperglycemia, inflammation, atherosclerosis, hypertension and more. Vasculature stability plays a critical role in the development of the disease. Discover the latest research on cardiovascular disease pathophysiology here.

Biomarkers for Type 2 Diabetes

Biomarkers can help understand chronic diseases and assist in risk prediction for prevention and early detection of diseases. Here is the latest research on biomarkers in type 2 diabetes, a disease in which the body is unable to produce or properly use insulin.

Cardiovascular Diseases: Risk Factors

Cardiovascular disease is a significant health concern. Risk factors include hypertension, obesity, dyslipidemia and smoking. Women who are postmenopausal are at an increased risk of heart disease. Here is the latest research for risk factors of cardiovascular disease.