PMID: 11311518Apr 20, 2001Paper

Cardiac apoptosis in burned rats with delayed fluid resuscitation

Burns : Journal of the International Society for Burn Injuries
G Q WangY L Chen

Abstract

Clinical and experimental studies have shown that delayed fluid resuscitation postburn decreases heart function. We hypothesized that apoptosis occurs in the cardiomyocyte in this condition. To investigate this hypothesis, rats were burned, fluid resuscitation was delayed, and the integrity of cardiac genomic DNA in the burned rats was determined with an LM-PCR Ladder Assay kit. DNA fragmentation shown as DNA ladders on gels, the hallmark of apoptosis, was found in the heart tissue of these rats. In the early phase of delayed fluid resuscitation, the nuclear factor kappa B (NF-kappa B) was examined using an electrophoretic mobility shift assay and was found to be activated. In comparison with burned rats with immediate fluid resuscitation, nitric oxide levels in hearts from burned rats with delayed fluid resuscitation were significantly lower (P<0.01). These results suggest that apoptosis may be an important pathway for cardiac injury, which may result from the activation of NF-kappa B and decreased nitric oxide levels.

References

Sep 1, 1991·The Journal of Burn Care & Rehabilitation·Z F XiaD N Herndon
Feb 1, 1995·Journal of Pediatric Surgery·E M ReynoldsD P Doody
Nov 1, 1996·Circulation Research·H Fliss, D Gattinger
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Citations

Dec 28, 2006·Apoptosis : an International Journal on Programmed Cell Death·G GravanteG Sconocchia
Jun 26, 2003·Experimental Eye Research·Yoshihiro TakamuraYoshio Akagi
Oct 15, 2003·Burns : Journal of the International Society for Burn Injuries·Andrew Rawlingson
Aug 21, 2003·Burns : Journal of the International Society for Burn Injuries·Xu-Lin ChenGuang-Qing Wang
Aug 13, 2008·The Journal of Trauma·Jia-Ping ZhangWen-Chang Wang
Jul 7, 2012·PloS One·Rong XiaoYue-sheng Huang

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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