PMID: 9539810May 16, 1998Paper

Cardiac arrest in rodents: maximal duration compatible with a recovery of neuronal activity

Proceedings of the National Academy of Sciences of the United States of America
S Charpak, E Audinat

Abstract

We report here that during a permanent cardiac arrest, rodent brain tissue is "physiologically" preserved in situ in a particular quiescent state. This state is characterized by the absence of electrical activity and by a critical period of 5-6 hr during which brain tissue can be reactivated upon restoration of a simple energy (glucose/oxygen) supply. In rat brain slices prepared 1-6 hr after cardiac arrest and maintained in vitro for several hours, cells with normal morphological features, intrinsic membrane properties, and spontaneous synaptic activity were recorded from various brain regions. In addition to functional membrane channels, these neurons expressed mRNA, as revealed by single-cell reverse transcription-PCR, and could synthesize proteins de novo. Slices prepared after longer delays did not recover. In a guinea pig isolated whole-brain preparation that was cannulated and perfused with oxygenated saline 1-2 hr after cardiac arrest, cell activity and functional long-range synaptic connections could be restored although the electroencephalogram remained isoelectric. Perfusion of the isolated brain with the gamma-aminobutyric acid A receptor antagonist picrotoxin, however, could induce self-sustained temporal lobe epil...Continue Reading

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Citations

May 8, 2001·Journal of Neuroscience Research·J J VielG J Brewer
Jan 29, 2008·Neuropharmacology·Konstantin-Alexander Hossmann
Jan 5, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ronald W H VerwerDick F Swaab
Oct 13, 2019·Experimental Physiology·Damian M Bailey
Apr 19, 2019·Nature·Zvonimir VrseljaNenad Sestan
Jan 9, 2019·Frontiers in Cellular Neuroscience·Elvira JuzekaevaRoustem Khazipov
Jul 23, 2021·Nature Reviews. Neuroscience·Stefano G DanieleNenad Sestan

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