Cardiac Arrest Induces Ischemic Long-Term Potentiation of Hippocampal CA1 Neurons That Occludes Physiological Long-Term Potentiation

Neural Plasticity
James E OrfilaNidia Quillinan

Abstract

Ischemic long-term potentiation (iLTP) is a form of synaptic plasticity that occurs in acute brain slices following oxygen-glucose deprivation. In vitro, iLTP can occlude physiological LTP (pLTP) through saturation of plasticity mechanisms. We used our murine cardiac arrest and cardiopulmonary resuscitation (CA/CPR) model to produce global brain ischemia and assess whether iLTP is induced in vivo, contributing to the functionally relevant impairment of pLTP. Adult male mice were subjected to CA/CPR, and slice electrophysiology was performed in the hippocampal CA1 region 7 or 30 days later. We observed increased miniature excitatory postsynaptic current amplitudes, suggesting a potentiation of postsynaptic AMPA receptor function after CA/CPR. We also observed increased phosphorylated GluR1 in the postsynaptic density of hippocampi after CA/CPR. These data support the in vivo induction of ischemia-induced plasticity. Application of a low-frequency stimulus (LFS) to CA1 inputs reduced excitatory postsynaptic potentials in slices from mice subjected to CA/CPR, while having no effects in sham controls. These results are consistent with a reversal, or depotentiation, of iLTP. Further, depotentiation with LFS partially restored induct...Continue Reading

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Citations

Mar 13, 2019·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Ivan GoussakovAlexander Drobyshevsky
Dec 26, 2018·Journal of Alzheimer's Disease : JAD·Iris EscobarMiguel A Perez-Pinzon
Oct 15, 2019·Frontiers in Cellular Neuroscience·Keren AltmanNicola Maggio
Aug 27, 2021·Neuroscience and Biobehavioral Reviews·Matthieu FaillotSuhan Senova
Sep 5, 2021·Neuromodulation : Journal of the International Neuromodulation Society·Fioravante CaponeVincenzo Di Lazzaro

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

Clampfit
GraphPad Prism

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