Cardiac Cx43 and ECM Responses to Altered Thyroid Status Are Blunted in Spontaneously Hypertensive versus Normotensive Rats

International Journal of Molecular Sciences
Matus SykoraN Tribulova

Abstract

Heart function and its susceptibility to arrhythmias are modulated by thyroid hormones (THs) but the responsiveness of hypertensive individuals to thyroid dysfunction is elusive. We aimed to explore the effect of altered thyroid status on crucial factors affecting synchronized heart function, i.e., connexin-43 (Cx43) and extracellular matrix proteins (ECM), in spontaneously hypertensive rats (SHRs) compared to normotensive Wistar Kyoto rats (WKRs). Basal levels of circulating THs were similar in both strains. Hyperthyroid state (HT) was induced by injection of T3 (0.15 mg/kg b.w. for eight weeks) and hypothyroid state (HY) by the administration of methimazol (0.05% for eight weeks). The possible benefit of omega-3 polyunsaturated fatty acids (Omacor, 200 mg/kg for eight weeks) intake was examined as well. Reduced levels of Cx43 in SHRs were unaffected by alterations in THs, unlike WKRs, in which levels of Cx43 and its phosphorylated form at serine368 were decreased in the HT state and increased in the HY state. This specific Cx43 phosphorylation, attributed to enhanced protein kinase C-epsilon signaling, was also increased in HY SHRs. Altered thyroid status did not show significant differences in markers of ECM or collagen depo...Continue Reading

References

Feb 1, 1991·The American Journal of Physiology·M A KarimA M Samarel
Apr 14, 2000·Circulation Research·J E SaffitzK A Yamada
Jun 18, 2002·Journal of Cellular and Molecular Medicine·N J Severs
Aug 8, 2002·Thyroid : Official Journal of the American Thyroid Association·W H Dillmann
Jul 5, 2003·Molecular and Cellular Biochemistry·Barbara Ziegelhöffer-MihalovicováHeinz-Gerd Zimmer
Feb 20, 2004·The Journal of Biological Chemistry·Xiaoyong BaoGuillermo A Altenberg
Oct 23, 2004·Circulation Research·Stephan B DanikDavid E Gutstein
Feb 6, 2007·Matrix Biology : Journal of the International Society for Matrix Biology·Sreerupa Ghose RoyArun Bandyopadhyay
Apr 4, 2008·American Journal of Physiology. Cell Physiology·Nagomi KurebayashiYasuo Ogawa
Jun 4, 2008·Cardiovascular Research·Nicholas J SeversStephen Rothery
Jan 7, 2009·Heart Failure Reviews·Wolfgang Dillmann
Oct 24, 2009·Vascular Pharmacology·Narcis TribulovaTomas Soukup
Dec 3, 2011·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Heather S Duffy
Feb 9, 2012·International Journal of Molecular Sciences·Miroslav BarancikAlbert Breier
Mar 20, 2013·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·H RauchováT Soukup
Oct 22, 2014·PloS One·Georges HajjeNassim Farès
Mar 4, 2015·Journal of Electrocardiology·Narcis TribulovaCsilla Viczenczova
Jan 23, 2016·Current Problems in Cardiology·Irwin Klein, Sara Danzi
Apr 18, 2017·Biochimica Et Biophysica Acta. Biomembranes·Joell L Solan, Paul D Lampe
May 14, 2017·Circulation. Arrhythmia and Electrophysiology·Nicolò SalvaraniStephan Rohr
Oct 31, 2017·Nutrients·Narcis TribulovaJan Slezak
Apr 21, 2018·Journal of the American College of Cardiology·Salman RazviGiorgio Iervasi

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Methods Mentioned

BETA
PCR
Assay
light microscopy

Software Mentioned

Omacor
Carestream Molecular Imaging

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