Cardiac-Gated En Face Doppler Measurement of Retinal Blood Flow Using Swept-Source Optical Coherence Tomography at 100,000 Axial Scans per Second

Investigative Ophthalmology & Visual Science
ByungKun LeeJ G Fujimoto

Abstract

To develop and demonstrate a cardiac gating method for repeatable in vivo measurement of total retinal blood flow (TRBF) in humans using en face Doppler optical coherence tomography (OCT) at commercially available imaging speeds. A prototype swept-source OCT system operating at 100-kHz axial scan rate was developed and interfaced with a pulse oximeter. Using the plethysmogram measured from the earlobe, Doppler OCT imaging of a 1.5- × 2-mm area at the optic disc at 1.8 volumes/s was synchronized to cardiac cycle to improve sampling of pulsatile blood flow. Postprocessing algorithms were developed to achieve fully automatic calculation of TRBF. We evaluated the repeatability of en face Doppler OCT measurement of TRBF in 10 healthy young subjects using three methods: measurement at 100 kHz with asynchronous acquisition, measurement at 100 kHz with cardiac-gated acquisition, and a control measurement using a 400-kHz instrument with asynchronous acquisition. The median intrasubject coefficients of variation (COV) of the three methods were 8.0%, 4.9%, and 6.1%, respectively. All three methods correlated well, without a significant bias. Mean TRBF measured at 100 kHz with cardiac-gated acquisition was 40.5 ± 8.2 μL/min, and the range ...Continue Reading

Citations

Aug 25, 2020·Translational Vision Science & Technology·Stephan SzegediLeopold Schmetterer
Mar 27, 2019·Therapeutic Advances in Ophthalmology·Petteri TeikariDan Milea

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