Cardiac hypertrophy reduction in SHR by specific silencing of myocardial Na(+)/H(+) exchanger

Journal of Applied Physiology
Mariela B NollyPatricio E Morgan

Abstract

We examined the effect of specific and local silencing of sodium/hydrogen exchanger isoform 1 (NHE1) with a small hairpin RNA delivered by lentivirus (L-shNHE1) in the cardiac left ventricle (LV) wall of spontaneously hypertensive rats, to reduce cardiac hypertrophy. Thirty days after the lentivirus was injected, NHE1 protein expression was reduced 53.3 ± 3% in the LV of the L-shNHE1 compared with the control group injected with L-shSCR (NHE1 scrambled sequence), without affecting its expression in other organs, such as liver and lung. Hypertrophic parameters as LV weight-to-body weight and LV weight-to-tibia length ratio were significantly reduced in animals injected with L-shNHE1 (2.32 ± 0.5 and 19.30 ± 0.42 mg/mm, respectively) compared with L-shSCR-injected rats (2.68 ± 0.06 and 21.53 ± 0.64 mg/mm, respectively). Histochemical analysis demonstrated a reduction of cardiomyocytes cross-sectional area in animals treated with L-shNHE1 compared with L-shSCR (309,81 ± 20,86 vs. 424,52 ± 21 μm(2), P < 0.05). Echocardiography at the beginning and at the end of the treatment showed that shNHE1 expression for 30 days induced 9% reduction of LV mass. Also, animals treated with L-shNHE1 exhibited a reduced LV wall thickness without cha...Continue Reading

References

Oct 22, 1979·The American Journal of Cardiology·E D Frohlich, R C Tarazi
Sep 1, 1987·The American Journal of Cardiology·D LevyW P Castelli
Nov 1, 1974·Circulation Research·S SenF M Bumpus
Jan 29, 1999·Physiological Reviews·B Swynghedauw
Mar 1, 2002·Journal of the American College of Cardiology·Metin Avkiran, Michael S Marber
Mar 30, 2002·Cardiovascular Research·María C Camilión de HurtadoHoracio E Cingolani
Feb 8, 2003·Hypertension·Horacio E CingolaniMaría C Camilión de Hurtado
May 7, 2003·Hypertension·Irene L EnnisHoracio E Cingolani
May 19, 2004·European Journal of Echocardiography : the Journal of the Working Group on Echocardiography of the European Society of Cardiology·Eduardo Manuel EscuderoAna Laura Tufare
Dec 13, 2005·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Manabu KokuboToyoaki Murohara
Jul 25, 2006·Journal of Molecular and Cellular Cardiology·Raúl A DulceMaría C Camilión de Hurtado
Mar 7, 2007·Circulation·Horacio E Cingolani, Irene L Ennis
Mar 16, 2007·Pflügers Archiv : European journal of physiology·Iwan A WilliamsDavid G Allen
Nov 27, 2007·Journal of Molecular Biology·Sudhiranjan GuptaSubha Sen
Mar 11, 2008·Journal of Molecular and Cellular Cardiology·Morris KarmazynSabzali Javadov
Apr 5, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Andreas S BarthEduardo Marbán
Apr 10, 2008·Journal of Hypertension·Jane-Lise Samuel, Bernard Swynghedauw
Apr 10, 2008·Journal of Hypertension·Claudia GiampietriElio Ziparo
Nov 4, 2010·Journal of Hypertension·Edward D FrohlichJavier Díez
Mar 2, 2011·Basic Research in Cardiology·Fatima MraicheLarry Fliegel
May 21, 2011·Journal of Applied Physiology·Patricio E MorganHoracio E Cingolani
Jun 11, 2011·Journal of Applied Physiology·Néstor G PérezHoracio E Cingolani
Dec 21, 2013·Journal of Molecular and Cellular Cardiology·M B NollyI L Ennis

❮ Previous
Next ❯

Citations

Feb 1, 2018·Toxicological Sciences : an Official Journal of the Society of Toxicology·Gustav HolmgrenJane Synnergren
Oct 17, 2016·Journal of the American Heart Association·María S BreaNéstor G Pérez
Mar 29, 2019·Journal of the American Heart Association·Alejandro Martín IbañezVerónica Celeste De Giusti
Oct 12, 2019·Hipertensión y riesgo vascular·A M Yeves, I L Ennis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

Cardiomegaly

Cardiomegaly, known as an enlarged heart, is a multifactorial disease with different pathophysiological mechanisms. Hypertension, pregnancy, exercise-induced and idiopathic causes are some mechanisms of cardiomegaly. Discover the latest research of cardiomegaly here.