Cardiac inflammation and diastolic dysfunction in hypercholesterolemic rabbits

PloS One
Walid NacharJean-Claude Tardif

Abstract

Left ventricular diastolic dysfunction (LVDD) is present in more than 50% of patients suffering from heart failure. LVDD animal models are limited and its underlying mechanisms remain largely unknown. Aortic valve stenosis (AVS) may cause LVDD, and we recently reported LVDD in an AVS rabbit model. Here we aimed to develop a rabbit model of LVDD without AVS. Rabbits were fed with a 0.5% cholesterol-enriched diet (n = 9) or normal diet (n = 8) until they developed LVDD defined by a value of the echocardiographic parameter E/Em ratio higher than the mean at baseline + 2SD. Rabbits were then fed a 0.2% cholesterol-enriched diet for 4 weeks (average total diet duration: 20 weeks). Detailed cardiac structure and function measurements were assessed by echocardiography at baseline, weeks 8, 12 and 14 to 20, when applicable. Histological analyses and RT-qPCR were performed on LV samples. The hypercholesterolemic diet induced LVDD without systolic dysfunction or AVS, as shown by multiple echocardiographic parameters, including early filling mitral peak velocity and deceleration rate, Em/Am ratio and E/Em ratio (all p<0.05), and by increased cardiac mRNA expression of brain natriuretic peptide (Bnp). Cardiac expression of mRNA for Nox2, V...Continue Reading

References

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Citations

May 18, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Mayarling Francisca TroncosoSergio Lavandero
Nov 10, 2021·Heart Failure Reviews·Chihiro MiyagiKiyotaka Fukamachi

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BETA
genetic modification
sedation
ELISA
PCR

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SAS
geNorm
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