Cardiac length dependence of force and force redevelopment kinetics with altered cross-bridge cycling

Biophysical Journal
Bishow B AdhikariD A Martyn

Abstract

We examined the influence of cross-bridge cycling kinetics on the length dependence of steady-state force and the rate of force redevelopment (k(tr)) during Ca(2+)-activation at sarcomere lengths (SL) of 2.0 and 2.3 microm in skinned rat cardiac trabeculae. Cross-bridge kinetics were altered by either replacing ATP with 2-deoxy-ATP (dATP) or by reducing [ATP]. At each SL dATP increased maximal force (F(max)) and Ca(2+)-sensitivity of force (pCa(50)) and reduced the cooperativity (n(H)) of force-pCa relations, whereas reducing [ATP] to 0.5 mM (low ATP) increased pCa(50) and n(H) without changing F(max). The difference in pCa(50) between SL 2.0 and 2.3 microm (Delta pCa(50)) was comparable between ATP and dATP, but reduced with low ATP. Maximal k(tr) was elevated by dATP and reduced by low ATP. Ca(2+)-sensitivity of k(tr) increased with both dATP and low ATP and was unaffected by altered SL under all conditions. Significantly, at equivalent levels of submaximal force k(tr) was faster at short SL or increased lattice spacing. These data demonstrate that the SL dependence of force depends on cross-bridge kinetics and that the increase of force upon SL extension occurs without increasing the rate of transitions between nonforce and ...Continue Reading

References

May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B Brenner, E Eisenberg
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B Brenner
Sep 1, 1986·The Journal of Physiology·Y E Goldman, R M Simmons
Jun 24, 2000·Circulation Research·M RegnierP B Chase
Jun 29, 2000·Annual Review of Biochemistry·M A Geeves, K C Holmes
Jan 12, 2002·Circulation Research·John P KonhilasPieter P de Tombe
Jun 14, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Jeffrey W HolmesGerd Hasenfuss
Dec 6, 2002·Pflügers Archiv : European journal of physiology·John P KonhilasPieter P de Tombe
Sep 10, 2003·The Journal of Physiology·Norio FukudaHenk Granzier

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Citations

Sep 27, 2006·The Journal of General Physiology·Julian E Stelzer, Richard L Moss
Dec 29, 2010·The Journal of General Physiology·Nicholas M P KingHenk Granzier
Jan 2, 2013·The Journal of General Physiology·Nima Milani-NejadPaul M L Janssen
Dec 15, 2007·Cardiovascular Research·Laurin M HanftKerry S McDonald
Jul 12, 2013·Proceedings. Biological Sciences·C David WilliamsThomas L Daniel
Aug 7, 2012·American Journal of Physiology. Heart and Circulatory Physiology·F Steven KorteMichael Regnier
Nov 13, 2012·American Journal of Physiology. Heart and Circulatory Physiology·John Jeshurun MichaelMurali Chandra
Dec 6, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Jitandrakumar R PatelMarion L Greaser
Mar 3, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Laurin M Hanft, Kerry S McDonald
Apr 5, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Gerrie P FarmanPieter P de Tombe
Feb 9, 2016·Archives of Biochemistry and Biophysics·Laurin M HanftKerry S McDonald
Feb 26, 2016·Frontiers in Physiology·Ranganath MamidiJulian E Stelzer
Dec 19, 2015·Journal of Molecular and Cellular Cardiology·Pieter P de Tombe, Henk E D J ter Keurs
Dec 15, 2015·Journal of Molecular and Cellular Cardiology·Daria Amiad Pavlov, Amir Landesberg
Mar 6, 2016·Archives of Biochemistry and Biophysics·King-Lun LiWenji Dong
Sep 22, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Stephen C KolwiczMichael Regnier
Jul 25, 2006·Journal of Molecular and Cellular Cardiology·Natalie S SchneiderTetsuya Matsuda
May 2, 2014·Journal of Molecular and Cellular Cardiology·Scott D LundyMichael Regnier
Sep 20, 2011·Journal of Molecular and Cellular Cardiology·F S KorteMichael Regnier
Oct 18, 2011·Journal of Molecular and Cellular Cardiology·Anthony J Baker
Dec 17, 2009·Journal of Molecular and Cellular Cardiology·Yin-Biao Sun, Malcolm Irving
Jan 8, 2010·Journal of Molecular and Cellular Cardiology·Pieter P de TombeThomas C Irving
Feb 14, 2012·Journal of Pharmacological and Toxicological Methods·Robert L Hamlin, Carlos del Rio
Jan 31, 2006·Biophysical Journal·Murali ChandraKenneth B Campbell
Sep 27, 2012·Biophysical Journal·Sampath K GollapudiMurali Chandra
Sep 6, 2014·Frontiers in Physiology·Styliani Vakrou, M Roselle Abraham
Dec 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Dennis M AbrahamHoward A Rockman
Oct 6, 2005·Journal of Muscle Research and Cell Motility·Franklin Fuchs, Donald A Martyn
Aug 19, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Catalina NormanJonathan P Davis
Nov 18, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·István Ferenc EdesZoltán Papp
Oct 18, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Bertrand Cw TannerPeter O Awinda
Jun 12, 2020·Frontiers in Physiology·Kerry S McDonaldKenneth S Campbell
Aug 3, 2019·American Journal of Physiology. Heart and Circulatory Physiology·Sherif M Reda, Murali Chandra

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