Cardiac Mesenchymal Stem Cells Proliferate Early in the Ischemic Heart

European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes
Christian KlopschGustav Steinhoff

Abstract

Cardiac mesenchymal stem cells (MSCs) could stimulate cell-specific regenerative mechanisms after myocardial infarction (MI) depending on spatial origin, distribution, and niche regulation. We aimed at identifying and isolating tissue-specific cardiac MSCs that could contribute to regeneration. Following permanent ligation of the left anterior descending coronary artery in rats (n = 16), early cardiac tissues and cardiac mononuclear cells (MNCs) were analyzed by immunohistology, confocal laser scanning microscopy, and flow cytometry, respectively. Early postischemic specific MSCs were purified by fluorescence-activated cell sorting, cultivated under standardized culture conditions, and tested for multipotent differentiation in functional identification kits. Cardiac MSC niches were detected intramyocardially in cell clusters after MI and characterized by positive expression for vimentin, CD29, CD44, CD90, CD105, PDGFRα, and DDR2. Following myocardial ischemia, proliferation was induced early and proliferation density was approximately 11% in intramyocardial MSC clusters of the peri-infarction border zone. Cluster sizes increased by 157 and 64% in the peri-infarction and noninfarcted areas of infarcted hearts compared with nonin...Continue Reading

References

Feb 1, 2003·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Gianluca RigatelliUgo Carraro
Jul 25, 2003·The Journal of Thoracic and Cardiovascular Surgery·Sunil RangappaJ Yasha Kresh
May 22, 2004·Stem Cells·Joachim OswaldCarsten Werner
Jul 16, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Edie C GoldsmithThomas K Borg
Sep 27, 2005·Physiological Reviews·Annarosa LeriPiero Anversa
Jul 21, 2006·Cardiovascular Research·Helge MöllmannAlbrecht Elsässer
Feb 3, 2007·Current Vascular Pharmacology·Elise M K Furfaro, Mohamed A Gaballa
Aug 2, 2008·Journal of the American College of Cardiology·Jing FangNora L Keenan
Oct 4, 2008·The Journal of International Medical Research·X-J YangW-P Jiang
Aug 5, 2010·Cell Stem Cell·Ahmed MohyeldinAlfredo Quiñones-Hinojosa
Mar 2, 2011·Cardiovascular Research·Signe CarlsonMark L Entman
Apr 19, 2011·Medical & Biological Engineering & Computing·Phil Barnett, Maurice J B van den Hoff
Dec 6, 2011·Cell Stem Cell·James J H ChongRichard P Harvey
Apr 28, 2012·Circulation Research·Tilanthi M JayawardenaVictor J Dzau
Aug 21, 2012·Journal of Cellular Biochemistry·Donald O FreytesGordana Vunjak-Novakovic
Sep 14, 2012·Journal of Cardiovascular Translational Research·Jeffrey R CrawfordMark L Entman
Oct 3, 2012·PloS One·Bram van WijkMaurice J B van den Hoff
Aug 12, 2014·Stem Cells and Development·Martin J HoogduijnLuc J W van der Laan
Sep 25, 2014·Science Signaling·David M Gonzalez, Damian Medici
May 21, 2015·Journal of Cellular and Molecular Medicine·Anna SkorskaRobert David

❮ Previous
Next ❯

Citations

May 13, 2018·Disease Models & Mechanisms·Christian KlopschGustav Steinhoff
Sep 12, 2018·Physiological Research·M Trávníčková, L Bačáková
Jul 9, 2020·International Journal of Molecular Sciences·Yi-Hsiung LinChung-Hwan Chen
Feb 29, 2020·Regenerative Therapy·Edward H NtegeYusuke Shimizu
Apr 30, 2021·Frontiers in Physiology·Maria Cimini, Raj Kishore

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiac Regeneration

Cardiac regeneration enables the repair of irreversibly damaged heart tissue using cutting-edge science, including stem cell and cell-free therapy. Discover the latest research on cardiac regeneration here.

Related Papers

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery
Xin TianLi Deng
© 2022 Meta ULC. All rights reserved