Cardiac repair in a murine model of myocardial infarction with human induced pluripotent stem cell-derived cardiomyocytes.

Stem Cell Research & Therapy
Xin JiangMing Dong

Abstract

Cellular replacement strategies using human induced pluripotent stem cells (iPSCs) and their cardiac derivatives are emerging as novel treatments for post-myocardial infarction (MI) heart failure (HF); however, the mechanism of recovery of heart function is not very clear. The purpose of this study was to investigate the efficiency of using highly purified human induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs) for myocardial repair in a mouse model of MI and to clarify the mechanism of recovery of heart function. Animals modelling MI were randomly assigned to receive direct intramyocardial injection of culture medium (MI group) or 4 × 105 iPS-CMs (cell group) at the infarct border zone. Left ventricle (LV) performance was assessed with serial cardiac electrophysiology and was measured 1, 2 and 4 weeks post-MI. Invasive LV pressure measurement was measured at 4 weeks and was followed by sacrifice for histological examination. Compared to the MI group, the left ventricle ejection fraction (LVEF), left ventricular internal diameter in end-diastole (LVIDd) and end-systole (LVIDs) and maximal positive and negative pressure derivative (±dP/dt) were significantly improved in the iPS-CM group at 4 weeks post-MI. Histologi...Continue Reading

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Citations

Mar 24, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Robin AugustinePablo Maureira
Jul 27, 2021·Advanced Healthcare Materials·Junnan TangJinying Zhang
Aug 28, 2021·Stem Cells·Ramana Vaka, Darryl R Davis

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Methods Mentioned

BETA
fluorescence microscopy
Flow cytometry
genetic modification

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