PMID: 8967455May 1, 1996Paper

Cardiac sodium channels expressed in a peripheral neurotumor-derived cell line, RT4-B8

The American Journal of Physiology
D ZengD A Hanck

Abstract

RT4-B is one of several cell lines derived from a multipotent stem cell line, RT4-AC, which originated from a rat peripheral neurotumor. Based on Northern blot and ribonuclease protection experiments, RT4-B8 cells have been proposed to express rat cardiac Na channel mRNA as the major isoform. We report here direct electrophysiological evidence that the expressed voltage-gated Na channels in the RT4-B8 cell line are of the cardiac phenotype with no evidence for subpopulations expressing other Na channel isoforms. Current activation half point (conductance) was -41 +/- 5 mV (n = 7) and the steady-state voltage-dependent availability half point was -89 +/- 1 mV. As expected for cardiac Na channels, the half concentration of block for tetrodotoxin block was 0.74 microM, for saxitoxin (STX) was 0.15 microM, and for the class 2B divalent cation Cd2+ was 67 microM. Block was well described by single-site dose-response relationships with no indication of a subpopulation with "neuronal" affinity. Single-channel conductance (140 mM Na+) was 10 pS and predicted the average number of channels open at peak Na current to be 3 channels/microns2. [3H]STX binding data were also consistent with a single population of low-affinity STX binding sit...Continue Reading

References

Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·W A Catterall, C S Morrow
Jun 11, 1992·Proceedings of the National Academy of Sciences of the United States of America·A L GeorgeM M Tamkun
Jan 1, 1990·Annual Review of Neuroscience·B A BarresD P Corey
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M R Freeman, N Sueoka

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Citations

May 19, 2011·Pflügers Archiv : European journal of physiology·Maximiliano Josè NigroJacopo Magistretti
Sep 27, 1996·The Journal of Biological Chemistry·B L Dias-KadambiK M Blumenthal
Aug 24, 2005·The European Journal of Neuroscience·Shao-Wu OuMasaki Kameyama
Dec 6, 2017·International Journal of Molecular Medicine·Jun WangYun-Jie Wang
May 30, 1998·Toxicon : Official Journal of the International Society on Toxinology·G J Kelso, K M Blumenthal
Nov 10, 2006·Toxicon : Official Journal of the International Society on Toxinology·Dorothy A Hanck, Michael F Sheets

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