Cardiac surgery with extracorporeal circulation: neutrophil transendothelial migration is mediated by beta1 integrin (CD29) in the presence of TNF-alpha

Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research
Martin ScholzAnton Moritz

Abstract

Cardiac surgery with extracorporeal circulation is associated with neutrophil activation, inflammation, and edema. Endothelial hyperpermeability elicited by the interaction of activated neutrophils and/or cytokines with endothelial cells may be critical in this regard. However, the immune and cellular mechanisms involved are not fully understood. Cocultures with human endothelial cells and neutrophils from cardiac surgery patients were used to evaluate the role of beta1 integrin activity and the proinflammatory cytokine tumor necrosis factor (TNF)-alpha in neutrophil transendothelial migration and in impairment of the integrity of endothelial cell-to-cell contacts. Blocking of CD29 (heavy chain of beta1 integrins) totally prevented neutrophil adhesion and transendothelial migration. Pretreatment of neutrophils with either a CD29-stimulating monoclonal antibody or the addition of TNF-alpha (0.1-10 U/ml) to the coculture failed to induce transendothelial migration. However, coculture of endothelial cells with CD29-stimulated neutrophils in the presence of 0.1-10 U/ml TNF-alpha strongly induced neutrophil transmigration. CD29/TNF-alpha-mediated transmigration was associated with intracellular redistribution of endothelial beta-cat...Continue Reading

References

Dec 1, 1990·Journal of Cellular Physiology·L S GibbsA B Malik
Feb 26, 1998·Anesthesiology·D N HarrisG M Bydder
Feb 4, 1999·The Annals of Thoracic Surgery·G Asimakopoulos, K M Taylor
Jul 31, 2002·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·M ScholzA Moritz

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Citations

Oct 11, 2007·Journal of Inflammation·Ulf Abdel-RahmanMartin Scholz
Jun 8, 2006·Medicinal Research Reviews·Martin ScholzJoachim Windolf
Sep 26, 2019·Communications Biology·Sayan GhoshDebasish Sinha
Jan 12, 2020·Communications Biology·Sayan GhoshDebasish Sinha

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