Cardiac troponin-I phosphorylation underlies myocardial contractile dysfunction induced by hypothermia rewarming.

American Journal of Physiology. Heart and Circulatory Physiology
Torkjel TveitaGary C Sieck

Abstract

Rewarming the intact heart after a period of hypothermia is associated with reduced myocardial contractility, decreased Ca2+ sensitivity, and increased cardiac troponin-I (cTnI) phosphorylation. We hypothesized that hypothermia/rewarming (H/R) induces left ventricular (LV) contractile dysfunction due to phosphorylation of cTnI at Ser23/24. To test this hypothesis, the response of wild-type mice (n = 7) to H/R was compared with transgenic (TG) mice expressing slow skeletal TnI (TG-ssTnI; n = 7) that lacks the Ser23/24 phosphorylation sites. Hypothermia was induced by surface cooling and maintained at 23-25°C for 3 h. Subsequently, the animals were rewarmed to 37°C. LV systolic and diastolic function was assessed using a 1.4 F pressure-volume Millar catheter introduced via the right carotid artery. At baseline conditions, there were no significant differences in LV systolic function between wild-type and TG-ssTnI mice, whereas measurements of diastolic function [isovolumic relaxation constant (τ) and end-diastolic pressure-volume relationship (EDPVR)] were significantly (P < 0.05) reduced in TG-ssTnI animals. Immediately after rewarming, significant differences between groups were found in cardiac output (CO; wild-type 6.6 ± 0.7 ...Continue Reading

References

Jul 1, 1988·The American Journal of Physiology·D D GlowerJ S Rankin
Sep 1, 1986·Journal of the Royal Society of Medicine·D Maclean
Mar 1, 1985·The Journal of Physiology·D G Stephenson, D A Williams
Mar 1, 1980·Anesthesiology·P A SteenJ D Michenfelder
Dec 8, 1998·Journal of Applied Physiology·T TveitaO Hevrøy
Mar 29, 2001·Intensive Care Medicine·B MégarbaneF G Brivet
Jun 18, 2004·Annals of the New York Academy of Sciences·Marius P SumandeaR John Solaro
Dec 24, 2004·Current Opinion in Critical Care·Samuel A Tisherman
Feb 8, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Timofei V KondratievTorkjel Tveita
Sep 2, 2006·Journal of Neuroscience Methods·Serge C Thal, Nikolaus Plesnila
Mar 27, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Jan Møller NielsenHans Erik Bøtker
Dec 29, 2007·Biochemical and Biophysical Research Communications·R John SolaroTomoyoshi Kobayashi
Oct 7, 2008·Cryobiology·Young-Soo HanGary C Sieck
Sep 1, 2009·The Journal of Experimental Biology·Thomas H MeekTheodore Garland
Dec 22, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Young-Soo HanGary C Sieck
Jun 30, 2010·The Journal of General Physiology·R John SolaroYunbo Ke
Oct 5, 2014·Cryobiology·Erik Sveberg DietrichsTorkjel Tveita
Mar 20, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Niccole SchaibleGary Sieck

❮ Previous
Next ❯

Citations

Mar 29, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Leslie M OgilvieJeremy A Simpson
Apr 29, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Young-Soo HanGary C Sieck

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR
electrophoresis

Software Mentioned

ADInstruments Chart DAQ
PVAN

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.