Cardiolipin synthase is associated with a large complex in yeast mitochondria.

The Journal of Biological Chemistry
M ZhaoMiriam L Greenberg

Abstract

The phospholipid cardiolipin (CL) is ubiquitous in eucaryotes and is unique in structure, subcellular localization, and potential function. Previous studies have shown that CL is associated with major respiratory complexes in the mitochondrial membrane. To determine whether CL biosynthesis requires the presence of intact respiratory complexes, we measured activity of CL synthase, which catalyzes the synthesis of CL from cytidine diphosphate diacylglycerol and phosphatidylglycerol, in Saccharomyces cerevisiae strains with genetic defects in the oxidative phosphorylation system. Assembly mutants of cytochrome oxidase had significantly reduced CL synthase activity, while assembly mutants of respiratory complex III and the F0F1-ATPase were less inhibited. To obtain further information on the activity of CL synthase, we purified the enzyme and compared the size of the catalytic protein with the functional molecular mass. The enzyme was solubilized by Triton X-100 from KSCN-extracted mitochondrial membranes of S. cerevisiae. The functional molecular mass of Triton-solubilized CL synthase, determined by radiation inactivation, was 150-240 kDa, indicating that the functional enzyme was a large complex. After partial purification, the e...Continue Reading

References

Jan 1, 1979·Analytical Biochemistry·E S Kempner, W Schlegel
Jan 1, 1992·Methods in Enzymology·M Schlame, K Y Hostetler
May 1, 1990·Trends in Biochemical Sciences·H WeissW Neupert
Sep 18, 1990·Biochimica Et Biophysica Acta·K T Tamai, M L Greenberg
Dec 15, 1990·The Biochemical Journal·M Schlame, B Rüstow
Jun 12, 1985·Biochimica Et Biophysica Acta·G Daum
Mar 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·C B Hirschberg, E P Kennedy
Apr 1, 1993·Journal of Bioenergetics and Biomembranes·N C Robinson

❮ Previous
Next ❯

Citations

May 9, 2000·Progress in Lipid Research·M SchlameM L Greenberg
Mar 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Mauro Serricchio, Peter Bütikofer
May 16, 2012·The Journal of Biological Chemistry·Michael A KiebishRichard W Gross
Dec 17, 2008·The Journal of Biological Chemistry·Uma D VempatiCarlos T Moraes
Dec 14, 2007·Journal of Lipid Research·Michael Schlame
Oct 8, 2013·Antioxidants & Redox Signaling·Giuseppe ParadiesGiuseppe Petrosillo
Nov 5, 2013·Chemistry and Physics of Lipids·Matthew G BaileSteven M Claypool
Apr 7, 2005·FEBS Letters·Marcin NowickiMargrit Frentzen
Mar 17, 2009·Free Radical Biology & Medicine·Valerian E KaganGrigory Borisenko
Aug 15, 2006·Biochimica Et Biophysica Acta·Guiling LiMiriam L Greenberg
Mar 21, 2006·Journal of Lipid Research·Biao LuKenneth R Feingold
Aug 5, 2004·The Journal of Biological Chemistry·Vishal M GohilMiriam L Greenberg
Oct 27, 2017·FEBS Letters·Writoban Basu BallVishal M Gohil
Jan 19, 2018·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Mauro SerricchioG Angus McQuibban
May 2, 1998·Analytical Biochemistry·M ZhaoM L Greenberg
Oct 10, 2021·Journal of Inherited Metabolic Disease·Jiajia Ji, Miriam L Greenberg

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.