Cardiomyocyte bridging between hearts and bioengineered myocardial tissues with mesenchymal transition of mesothelial cells

The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation
Hidekazu SekineTeruo Okano

Abstract

For the reconstruction of 3-dimensional (3D) tissues, we exploited an original method of tissue engineering that layers individual cell sheets harvested from temperature-responsive culture dishes. Stacked cardiomyocyte sheets demonstrated electrical and morphologic communication, resulting in synchronously beating myocardial tissue. When these bioengineered 3D tissue grafts are transplanted onto damaged hearts, gap junction communication between graft and host is likely critical for synchronized beating and functional improvement. In this study, these graft-to-heart morphologic communications were examined. Neonatal rat cardiomyocyte sheets were harvested from temperature-responsive culture dishes and layered to create 3D tissues. These constructs were then transplanted onto infarcted rat hearts. Histologic analyses and transmission electron microscopy (TEM) were performed to examine morphologic communications. The passage of small molecules through functional gap junctions was also detected using a dye-transfer assay. Transplanted cardiomyocytes bridged between the grafts and hearts in intact areas. Connexin-43 staining and TEM revealed the existence of gap junctions and intercalated disks between the bridging cardiomyocytes. ...Continue Reading

References

Jan 1, 1995·Acta Anatomica·E D Hay
Jan 1, 1996·Acta Anatomica·C BirchmeierB Brand-Saberi
Nov 24, 1999·Circulation·R K LiT M Yau
Feb 24, 2001·Lancet·P MenaschéJ P Marolleau
Feb 9, 2002·Journal of Biomedical Materials Research·Tatsuya ShimizuTeruo Okano
Jan 31, 2003·The New England Journal of Medicine·María Yáñez-MóManuel López-Cabrera
Apr 18, 2003·Biomaterials·Tatsuya ShimizuTeruo Okano
May 6, 2003·Circulation Research·Michael RubartLoren J Field
Jul 13, 2004·Lancet·A Mathur, J F Martin
Sep 17, 2004·The New England Journal of Medicine·Kohji NishidaYasuo Tano
Dec 21, 2004·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Masaki HamamotoSoichiro Kitamura

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Citations

Mar 6, 2012·Cellular and Molecular Life Sciences : CMLS·Andre AlconYibing Qyang
Nov 19, 2011·Cell and Tissue Research·Hidekazu SekineTeruo Okano
Jun 1, 2012·Journal of Cardiovascular Translational Research·Niall G Campbell, Ken Suzuki
Oct 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hannah SongPeter W Zandstra
Dec 1, 2012·Stem Cells Translational Medicine·Yuji HaraguchiTeruo Okano
Mar 19, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Katsuhisa MatsuuraTeruo Okano
Jul 22, 2011·Stem Cells International·Outi M VilletAri Harjula
Aug 22, 2006·Expert Opinion on Biological Therapy·Harald C Ott, Doris A Taylor
Nov 17, 2009·Current Opinion in Biotechnology·Doris A Taylor
Nov 17, 2007·Advanced Drug Delivery Reviews·Shinako MasudaTeruo Okano
Sep 1, 2007·Biomaterials·Joseph YangTeruo Okano
Nov 27, 2007·Journal of Tissue Engineering and Regenerative Medicine·H JawadA R Boccaccini
Oct 15, 2013·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·David A DunnElizabeth A Lipke
Jan 29, 2014·Journal of Tissue Engineering and Regenerative Medicine·Ryohei TakeuchiTeruo Okano
Aug 8, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Joseph YangTeruo Okano
Dec 22, 2010·The Annals of Thoracic Surgery·Shigeru MiyagawaSawa Kostin
Oct 19, 2011·Cardiology Research and Practice·Yuji HaraguchiTeruo Okano
Nov 5, 2014·PloS One·Alexander WeymannGabor Szabo
Nov 7, 2012·Cell Transplantation·Hadhami HamdiPhilippe Menasché
Sep 25, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Jun FujitaKeiichi Fukuda
Jan 10, 2020·Stem Cell Research & Therapy·Rui GuoYunpeng Ling
Jul 14, 2007·The International Journal of Artificial Organs·S E Herrick, S E Mutsaers
May 11, 2010·Science and Technology of Advanced Materials·Jun Kobayashi, Teruo Okano
Dec 31, 2020·International Journal of Molecular Sciences·Hidekazu Sekine, Teruo Okano
Jul 6, 2006·Circulation·Hidekazu SekineTeruo Okano
Jan 19, 2021·ACS Biomaterials Science & Engineering·Mattia SponchioniDavide Moscatelli

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