Cardiomyocyte grafting for cardiac repair: graft cell death and anti-death strategies

Journal of Molecular and Cellular Cardiology
M ZhangC E Murry

Abstract

M. Zhang, D. Methot, V. Poppa, Y. Fujio, K. Walsh and C. E. Murry. Cardiomyocyte Grafting for Cardiac Repair: Graft Cell Death and Anti-Death Strategies. Journal of Molecular and Cellular Cardiology (2001) 33, 907-921. Recent studies indicate that cardiomyocyte grafting forms new myocardium in injured hearts. It is unknown, however, whether physiologically significant amounts of new myocardium can be generated. Pilot experiments showed that death of grafted rat neonatal cardiomyocytes limited formation of new myocardium after acute cryoinjury. Time-course studies showed that, at 30 min after grafting, only 1.8(+/-0.4)% of graft cells were TUNEL-positive. At 1 day, however, TUNEL indices increased to 32.1(+/-3.5)% and remained high at 4 days, averaging 9.8(+/-3.8)%. By 7 days, TUNEL decreased to 1.0(+/-0.2)%. Electron microscopy revealed that dead cells had features of both irreversible ischemic injury and apoptosis. To test whether ischemia contributed to poor graft survival, grafts were placed into vascularized 2-week-old cardiac granulation tissue or normal myocardium. TUNEL indices were reduced by 53% and 86%, respectively. Adenoviral infection of graft cells with the cytoprotective kinase Akt, or constitutively active Akt, ...Continue Reading

References

Aug 1, 1992·Journal of Molecular and Cellular Cardiology·D M YellonR Ferrari
Oct 1, 1995·The Journal of Clinical Investigation·G Y KohL J Field
Jan 1, 1995·Journal of Molecular and Cellular Cardiology·R Mestril, W H Dillmann
Feb 1, 1996·Circulation Research·R K LiM K Mohabeer
Sep 1, 1996·The Annals of Thoracic Surgery·R K LiJ Ivanov
Dec 1, 1996·The Journal of Clinical Investigation·C E MurryS D Hauschka
Jul 22, 1998·Circulation Research·M H Soonpaa, L J Field
Aug 1, 1998·Circulation Research·I J Benjamin, D R McMillan
Nov 3, 1998·The EMBO Journal·M JäätteläM Egeblad
Nov 6, 1998·Science·J A ThomsonJ M Jones
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·M J ShamblottJ D Gearhart
Apr 13, 1999·Journal of Molecular and Cellular Cardiology·R K LiD A Mickle
Sep 10, 1999·Annals of Medicine·M Jäättelä
Oct 3, 1999·The Journal of Thoracic and Cardiovascular Surgery·T SakaiT M Yau
Dec 2, 1999·Genes & Development·S R DattaM E Greenberg

❮ Previous
Next ❯

Citations

Mar 6, 2012·Cellular and Molecular Life Sciences : CMLS·Andre AlconYibing Qyang
Nov 19, 2011·Cell and Tissue Research·Hidekazu SekineTeruo Okano
Oct 7, 2008·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Hiroshi KobayashiTeruo Okano
Apr 10, 2007·Annals of Biomedical Engineering·Bryce H DavisDoris A Taylor
Apr 29, 2005·The International Journal of Cardiovascular Imaging·Kevin S CahillGlenn A Walter
May 22, 2009·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Hui QiaoRong Zhou
Jul 27, 2007·Science in China. Series C, Life Sciences·Sheng YangJacques Menetrey
Jan 5, 2013·In Vitro Cellular & Developmental Biology. Animal·Harsha PawaniDeepa Bhartiya
Mar 1, 2012·Cell Stress & Chaperones·Mohammad MohammadzadehMehryar Habibi Roudkenar
Jun 19, 2010·Journal of Cardiovascular Translational Research·Dinender K Singla
Mar 23, 2011·Journal of Cardiovascular Translational Research·Erik WillemsMark Mercola
Nov 18, 2011·Journal of Cardiovascular Translational Research·Jay H Traverse
Oct 12, 2012·Journal of Cardiovascular Translational Research·Kohei Inagawa, Masaki Ieda
May 26, 2005·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Paul W M FedakRen-Ke Li
Aug 6, 2004·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Joseph C WuSanjiv S Gambhir
Jul 21, 2004·Pathologie-biologie·M Pucéat
Jun 6, 2003·Journal of Molecular and Cellular Cardiology·Thorsten ReffelmannRobert A Kloner
Jun 6, 2003·Journal of Molecular and Cellular Cardiology·Mu YaoRobert A Kloner
Aug 11, 2012·Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan·Wei CaoJie Wang
Mar 12, 2003·Journal of the American College of Cardiology·Rutger J HassinkPieter A Doevendans
Apr 8, 2003·Journal of the American College of Cardiology·Elina MinamiCharles E Murry
Apr 8, 2003·Journal of the American College of Cardiology·Philippe MenaschéDenis Duboc
Nov 20, 2002·Journal of the American College of Surgeons·Christopher D RaeburnAlden H Harken
Jul 9, 2005·Nature Biotechnology·Michael A Laflamme, Charles E Murry
Dec 21, 2006·Nature Clinical Practice. Cardiovascular Medicine·Eric D Adler, Thomas M Maddox
Nov 21, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Thomas LaumonierJacques Menetrey
Aug 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Lauran R MaddenBuddy D Ratner
Feb 11, 2009·Antioxidants & Redox Signaling·Buddhadeb DawnEwa K Zuba-Surma
Feb 11, 2009·Antioxidants & Redox Signaling·Mohammad N Jameel, Jianyi Zhang
Feb 11, 2009·Antioxidants & Redox Signaling·Dinender K Singla
Dec 15, 2011·Stem Cells and Development·Samantha Licy StubbsRodney James Dilley

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis